Fig.8. Modeling SI-ALI Using Patient-Derived Lung Organoids and
Verification of Genes Associated with SI-ALI.
- Representative picture of the growth of lung organoid. Scale bars, 50
μm.
- Representative immunofluorescence images of 3D lung organoid in
Matrigel (nuclei stained with DAPI in blue, E-cadherin in green, SFTPC
in red). Scale bars, 50 μm.
- Immunofluorescence micrographs of lung organoid derived epithelial
cells on the lung chip (nuclei with DAPI in blue, E-cadherin in green,
SFTPC in red). Scale bars, 75 μm.
- Differential expression of genes involved in inflammation and
apoptosis within the epithelial or endothelial layers of the organoid
on a chip model , under varying conditions of smoke exposure and
treatment. Three separate studies were executed on the chip, with the
results expressed as mean ± standard deviation (SD).
- Quantitative analysis of mRNA expression levels for the identified
representative molecules.