2.8 Enzyme-linked immunosorbent assays (ELISA)
To evaluate the secretion of IL-6, TNF-α and IL-1β from the vascular
layer of the chip, culture medium was harvested from the top layer,
centrifuged at 12,000 rpm for 10 minutes at 4°C, and the supernatant
collected. The quantification of IL-6, TNF-α, and IL-1β was carried out
using human ELISA kits (Biolegend, USA) according to the manufacturer’s
instructions.