2.10 Analysis of THP-1 adhesion on the endothelium in the lung chip
THP-1 cell adhesion on the endothelium within the lung chip was evaluated by pre-treating the cells with a live/dead staining reagent (Thermo, USA). Following this, THP-1 cells were introduced to the upper layer of the chip. Four hours post-smoke exposure, THP-1 cell aggregation in both control and experimental group (mostly stained in green) using fluorescence microscope. Image quantification was executed using ImageJ software.