2.10 Analysis of THP-1 adhesion on the endothelium in the lung
chip
THP-1 cell adhesion on the endothelium within the lung chip was
evaluated by pre-treating the cells with a live/dead staining reagent
(Thermo, USA). Following this, THP-1 cells were introduced to the upper
layer of the chip. Four hours post-smoke exposure, THP-1 cell
aggregation in both control and experimental group (mostly stained in
green) using fluorescence microscope. Image quantification was executed
using ImageJ software.