Fig.8. Modeling SI-ALI Using Patient-Derived Lung Organoids and Verification of Genes Associated with SI-ALI.
  1. Representative picture of the growth of lung organoid. Scale bars, 50 μm.
  2. Representative immunofluorescence images of 3D lung organoid in Matrigel (nuclei stained with DAPI in blue, E-cadherin in green, SFTPC in red). Scale bars, 50 μm.
  3. Immunofluorescence micrographs of lung organoid derived epithelial cells on the lung chip (nuclei with DAPI in blue, E-cadherin in green, SFTPC in red). Scale bars, 75 μm.
  4. Differential expression of genes involved in inflammation and apoptosis within the epithelial or endothelial layers of the organoid on a chip model , under varying conditions of smoke exposure and treatment. Three separate studies were executed on the chip, with the results expressed as mean ± standard deviation (SD).
  5. Quantitative analysis of mRNA expression levels for the identified representative molecules.