2.7 Fluorescence imaging of coumarin 6-loaded beta-CD nanocage
The preparation of coumarin 6-loaded beta-CD nanocages followed a similar procedure to that of DOX-loaded beta-CD nanocages. SKOV-3 cells were seeded in 6-well plates at a density of 1×106cells/well in 2 ml of culture medium. After 12 hours of culture, the cells were pretreated with pH 4.5 PBS (1 ml), 5 mM GSH (1 ml), and pH 7.4 PBS (1 ml) for 2 hours. Subsequently, 1 ml of fresh culture medium containing coumarin 6-loaded beta-CD nanocages with an equivalent coumarin 6 concentration of 112.5 ng/mL was added, and the cells were incubated for 3 and 6 hours, respectively. Finally, the cells were washed thrice with PBS, and fluorescence images were captured using an Olympus IX 73 microscope.