2.4 Preparation of DOX-loaded beta-CD nanocage
Beta-CD nanocages (100 mg) were dissolved in 5 ml of PBS solution (pH 7.4). DOX (100 mg) was dissolved in 1 ml of DMSO and added to the beta-CD nanocage solution using a microsyringe. The solution was stirred in the dark for 12 hours. Subsequently, the solution was transferred to an Amicon centrifuge tube (MWCO 3000, Merck Millipore, USA) for concentration and washed three times with PBS to remove unloaded DOX. The final residue was freeze-dried.