2.6 Biocompatibility of beta-CD nanocage and cell killing effect of DOX-loaded beta-CD nanocage
SKOV-3 cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 μg/ml penicillin (Sigma-Aldrich), and 100 μg/ml streptomycin (Sigma-Aldrich) at 37 °C with 5% CO2. The cells were seeded in a 96-well plate at a density of 10,000 cells/well in 100 μl of culture medium. After 12 hours, the culture medium was replaced with 100 μl of fresh culture medium containing different concentrations of beta-CD nanocages for 12 hours. Cell viability was assessed using a CCK-8 assay (Solarbio).
For the cell killing rate test, SKOV-3 cells in a 96-well plate were pretreated with pH 4.5 PBS (100 μl), 5 mM GSH (100 μl), and pH 7.4 PBS (100 μl) for 2 hours. Subsequently, 100 μl of fresh culture medium containing different concentrations of DOX-loaded beta-CD nanocages was added and incubated for 12 hours. Cell viability was evaluated using a CCK-8 assay.