2.4 Preparation of DOX-loaded beta-CD nanocage
Beta-CD nanocages (100 mg) were dissolved in 5 ml of PBS solution (pH
7.4). DOX (100 mg) was dissolved in 1 ml of DMSO and added to the
beta-CD nanocage solution using a microsyringe. The solution was stirred
in the dark for 12 hours. Subsequently, the solution was transferred to
an Amicon centrifuge tube (MWCO 3000, Merck Millipore, USA) for
concentration and washed three times with PBS to remove unloaded DOX.
The final residue was freeze-dried.