2.8 Flow cytometry analysis of coumarin 6-loaded beta-CD
nanocage
SKOV-3 cells were seeded in 6-well plates at a density of
1×106 cells/well in 2 ml of culture medium. After 12
hours of culture, the cells were pretreated with pH 4.5 PBS (1 ml), 5 mM
GSH (1 ml), and pH 7.4 PBS (1 ml) for 2 hours. Subsequently, 1 ml of
fresh culture medium containing coumarin 6-loaded beta-CD nanocages with
an equivalent coumarin 6 concentration of 112.5 ng/mL was added, and the
cells were incubated for 3 and 6 hours, respectively. Finally, the cells
were collected by trypsin digestion and analyzed using a flow cytometer
(COULTER EPICS XL, Beckman, USA). The data were analyzed using FlowJo
software.