Role of Macrophage-Derived Ti-EVs in the Progression of High
Myopic Cataracts
A total of 6,161 Ti-EVs were classified as Macrophage-derived based on
their protein expression profiles. Initially divided into 11 subclusters
(Table S4), these were later consolidated into 9 Macrophage subclusters
with distinct functions: Healing, Eye morphogenesis, Cell recognition,
DC APC, Cell killing, T cell differentiation, Immunoglobulin, Protein
localization, and Adhesion (Figure 4A; Table S5). In terms of
prevalence, Macrophage-derived Ti-EVs were more common in senile
cataracts, largely due to differences in sample size (Figure 4C).
Additionally, the subclusters were evenly distributed across all
samples, suggesting low sample heterogeneity (Figure 4D). Notably, the
Eye morphogenesis, Cell killing, Adhesion, and T cell differentiation
subclusters exhibited higher Ro/e values in pathological cataracts
(Figure 4E), indicating that these subclusters may play a role in
contributing the development of high myopic cataracts.