EV Characterization
TEM revealed that both groups of separated EVs exhibited a cup-like structure (Figure 1B). NanoFlow CytoMetry showed that the particle sizes of EVs from both groups were distributed between 50-150 nm, with no significant difference in EVs concentration (Figure 1C-E). We quantified the tetraspanins subpopulations (CD9, CD81, CD63) in both EV samples, and the figure showed representative distribution of the CD63-positive subpopulation (Figure 1F). Both groups exhibited corresponding positive EV subpopulations. These findings confirm the successful separation of EVs.