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Figure 1 Collection of Lens Capsules, Incubation, Isolation and Characterization of Ti-EVs from Lens Capsules. A. Schematic illustration of collection of lens capsules during cataract surgery and the following incubation and isolation of Ti-EVs from the lens capsules. B. The structure of the separated EVs was examined using TEM. Scale bar represented 200 nm in figures. C. The size distribution of separated EVs demonstrated by nFCM. D. The concentration of separated EVs. E. The characters of the separated EVs were examined using nFCM with specific antibodies against EV membrane‐specific proteins CD63. Unpaired t-test. F. Proportion of EV membrane‐specific proteins in both groups, including CD9, CD81, CD63.
Figure 2 Immune Cell Derived EVs in the Progression of High Myopic Cataract. A. Heatmap displaying differential proteins in EVs derived from the lens tissues of age-related and high myopic cataract. B. Dot plot showing the ranking of differential proteins in EVs from the lens tissues. C. Bar plot illustrating the expression levels (TMM Normalized) of ABCG2, ERBB2, and CD86 in EVs. D. Heatmap presenting the expression levels of statistically significant differential proteins in EVs. E. Overview of EVs derived from the lens tissues of age-related and high myopic cataract. F. Overview of the origins of EVs derived from the lens tissues, as determined by PBA. G. Panorama of annotated EVs subclusters derived from the lens tissues. H. Heatmap showing the expression of marker proteins in each subcluster. I. Proportional chart depicting the contribution of EVs from different cell sources in age-related and high myopic cataract. J. Proportional chart showing the contribution of EVs from different cell sources across individual samples. K. Distribution of Ro/e values for EV subclusters in age-related and high myopic cataract. L. Expression levels of ABCG2, ERBB2, and CD86 across different EVs.
Figure 3 Macrophage-Derived EVs Synergistically Promote the Development of High Myopic Cataracts via AQP1. A. UMAP plot illustrating the global distribution of immune cell derived EVs. B. Dot plot showing marker proteins for different immune cell-derived EV subclusters. C. Heatmap displaying the highly expressed proteins and their associated pathways in immune cell derived EV subclusters. D. Proportional chart representing the distribution of immune cell derived EVs in age-related and high myopic cataract. E. Proportional chart showing the contribution of immune cell derived EVs in individual samples F. Distribution of Ro/e values for immune cell derived EV subclusters in age-related and high myopic cataract. G. Dot plot comparing shared protein expression between single EVs and bulk proteomics. H. Distribution of immune EV subclusters across age-related and high myopic cataract samples. I. Correlation of immune EV subclusters in age-related and high myopic cataract using deconvolution. J. Dot plot showing the correlation between AQP1 expression and immune cell derived EVs.
Figure 4 Macrophage-Derived EVs Facilitate High Myopic Cataract Development through Eye Morphogenesis. A. UMAP plot illustrating the distribution of macrophage-derived EV subclusters. B. Heatmap showing the highly expressed proteins and associated pathways in macrophage-derived EV subclusters. C. Proportional chart showing the distribution of macrophage-derived EVs in age-related and high myopic cataract. D. Proportional chart illustrating the contribution of macrophage-derived EVs across individual samples. E. Distribution of Ro/e values for macrophage-derived EV subclusters in age-related and high myopic cataract.
Figure 5 AQP1 in Macrophage-Derived EVs Synergistically Promotes High Myopic Cataract Progression via Eye Morphogenesis . A. Volcano plot illustrating differential proteins in macrophage-derived EV subclusters in age-related and high myopic cataract. B. Correlation pie chart for macrophage-derived EV subclusters. C. Distribution of different functional subclusters of macrophage-derived EVs in age-related and high myopic cataract. D. Violin plot showing the expression levels of AQP1 in different functional subclusters of macrophage-derived EVs.