Figure Legend
Figure 1 Collection of Lens Capsules, Incubation, Isolation and
Characterization of Ti-EVs from Lens Capsules. A. Schematic
illustration of collection of lens capsules during cataract surgery and
the following incubation and isolation of Ti-EVs from the lens capsules.
B. The structure of the separated EVs was examined using TEM. Scale bar
represented 200 nm in figures. C. The size distribution of separated EVs
demonstrated by nFCM. D. The concentration of separated EVs. E. The
characters of the separated EVs were examined using nFCM with specific
antibodies against EV membraneāspecific proteins CD63. Unpaired t-test.
F. Proportion of EV membraneāspecific proteins in both groups, including
CD9, CD81, CD63.
Figure 2 Immune Cell Derived EVs in the Progression of High
Myopic Cataract. A. Heatmap displaying differential proteins in EVs
derived from the lens tissues of age-related and high myopic cataract.
B. Dot plot showing the ranking of differential proteins in EVs from the
lens tissues. C. Bar plot illustrating the expression levels (TMM
Normalized) of ABCG2, ERBB2, and CD86 in EVs. D. Heatmap presenting the
expression levels of statistically significant differential proteins in
EVs. E. Overview of EVs derived from the lens tissues of age-related and
high myopic cataract. F. Overview of the origins of EVs derived from the
lens tissues, as determined by PBA. G. Panorama of annotated EVs
subclusters derived from the lens tissues. H. Heatmap showing the
expression of marker proteins in each subcluster. I. Proportional chart
depicting the contribution of EVs from different cell sources in
age-related and high myopic cataract. J. Proportional chart showing the
contribution of EVs from different cell sources across individual
samples. K. Distribution of Ro/e values for EV subclusters in
age-related and high myopic cataract. L. Expression levels of ABCG2,
ERBB2, and CD86 across different EVs.
Figure 3 Macrophage-Derived EVs Synergistically Promote the
Development of High Myopic Cataracts via AQP1. A. UMAP plot
illustrating the global distribution of immune cell derived EVs. B. Dot
plot showing marker proteins for different immune cell-derived EV
subclusters. C. Heatmap displaying the highly expressed proteins and
their associated pathways in immune cell derived EV subclusters. D.
Proportional chart representing the distribution of immune cell derived
EVs in age-related and high myopic cataract. E. Proportional chart
showing the contribution of immune cell derived EVs in individual
samples F. Distribution of Ro/e values for immune cell derived EV
subclusters in age-related and high myopic cataract. G. Dot plot
comparing shared protein expression between single EVs and bulk
proteomics. H. Distribution of immune EV subclusters across age-related
and high myopic cataract samples. I. Correlation of immune EV
subclusters in age-related and high myopic cataract using deconvolution.
J. Dot plot showing the correlation between AQP1 expression and immune
cell derived EVs.
Figure 4 Macrophage-Derived EVs Facilitate High Myopic Cataract
Development through Eye Morphogenesis. A. UMAP plot illustrating the
distribution of macrophage-derived EV subclusters. B. Heatmap showing
the highly expressed proteins and associated pathways in
macrophage-derived EV subclusters. C. Proportional chart showing the
distribution of macrophage-derived EVs in age-related and high myopic
cataract. D. Proportional chart illustrating the contribution of
macrophage-derived EVs across individual samples. E. Distribution of
Ro/e values for macrophage-derived EV subclusters in age-related and
high myopic cataract.
Figure 5 AQP1 in Macrophage-Derived EVs Synergistically Promotes
High Myopic Cataract Progression via Eye Morphogenesis . A. Volcano plot
illustrating differential proteins in macrophage-derived EV subclusters
in age-related and high myopic cataract. B. Correlation pie chart for
macrophage-derived EV subclusters. C. Distribution of different
functional subclusters of macrophage-derived EVs in age-related and high
myopic cataract. D. Violin plot showing the expression levels of AQP1 in
different functional subclusters of macrophage-derived EVs.