EV Characterization
TEM revealed that both groups of separated EVs exhibited a cup-like
structure (Figure 1B). NanoFlow CytoMetry showed that the particle sizes
of EVs from both groups were distributed between 50-150 nm, with no
significant difference in EVs concentration (Figure 1C-E). We quantified
the tetraspanins subpopulations (CD9, CD81, CD63) in both EV samples,
and the figure showed representative distribution of the CD63-positive
subpopulation (Figure 1F). Both groups exhibited corresponding positive
EV subpopulations. These findings confirm the successful separation of
EVs.