Role of Macrophage-Derived Ti-EVs in the Progression of High Myopic Cataracts
A total of 6,161 Ti-EVs were classified as Macrophage-derived based on their protein expression profiles. Initially divided into 11 subclusters (Table S4), these were later consolidated into 9 Macrophage subclusters with distinct functions: Healing, Eye morphogenesis, Cell recognition, DC APC, Cell killing, T cell differentiation, Immunoglobulin, Protein localization, and Adhesion (Figure 4A; Table S5). In terms of prevalence, Macrophage-derived Ti-EVs were more common in senile cataracts, largely due to differences in sample size (Figure 4C). Additionally, the subclusters were evenly distributed across all samples, suggesting low sample heterogeneity (Figure 4D). Notably, the Eye morphogenesis, Cell killing, Adhesion, and T cell differentiation subclusters exhibited higher Ro/e values in pathological cataracts (Figure 4E), indicating that these subclusters may play a role in contributing the development of high myopic cataracts.