Synergistic Role of Macrophage-Derived Ti-EVs and AQP1 in Promoting Eye Morphogenesis
Our findings indicated that the Eye morphogenesis cluster was characterized by high expression of HAVCR1, CPM, and MRC1; the Cell killing cluster by CDH15, PCDH1, MICA, and APOE; the Adhesion cluster by PCDH8, CDH3, CLDN10, VCAM1, and CDH11; and the T cell differentiation cluster by CD28 and CD80 (Figure 5A). Deconvolution analysis revealed a significant association among the Eye morphogenesis, Cell killing, and AQP1 subclusters (Figure 5B; Table S6). Additionally, Eye morphogenesis and Cell killing subclusters were more frequently observed in high myopic cataracts, suggesting a potential cooperative mechanism (Figure 5C). To further investigate the interactions between the AQP1 and Macrophage clusters, we analyzed AQP1 expression within macrophage subclusters and found it to be significantly elevated in the Eye morphogenesis cluster, suggesting that the Eye morphogenesis cluster may work in concert with AQP1, potentially driving the progression of high myopic cataracts through this pathway.