Proteomic analysis of HIIT-regulated activation of spinal
microglia in OA rats
The protein sequencing approach was used to analyse the gene
transcriptome between OA and OA+HIIT rats to elucidate the mechanisms of
action by which HIIT would relieve OA-induced pain. A total of 32 genes
showed significant differences, of which 10 genes were up-regulated and
22 genes were down-regulated when compared OA+HIIT with OA group (Figure
5A, E). PPI network shows the interactions between different proteins
(Figure 5B). KEGG pathway analysis was shown in Figure 5C-D, in which
Jak2/Stat3 signaling pathway is closer to HIIT treatment. The qRT-PCR
analysis verified down-regulation of genes related to HIIT, with the
expression levels of Stat3, Stat1, Jak2, and Grb2 significantly
down-regulated in the OA+HIIT group compared with the OA group (P < 0.05, Figure 5F).