HIIT reversed microglial polarization and mitigated inflammatory
responses in OA rats
TMEM119 was a key indicator of microgliosis. We respectively detected
TMEM119 in the microglia by immunofluorescence in control, OA, and
OA+HIIT rats. The expression level of Tmem119 was significantly higher
in OA rats than in control rats, and HIIT decreased its expression level
(P < 0.001, Figure 4A). In addition, we detected the
expression of CD68 and CD163 to distinguish pheno-typic transformations
of microglia. The flow cytometry results showed that the expression
level of CD68 was higher in the OA group than in the control group.
However, CD163 was significantly lower in the OA group compared with the
control group, and the expression level of CD163 was higher in the
OA+HIIT group than in the OA group (P < 0.01, Figure
4B). Moreover, OA+HIIT exhibited decreased expression levels of Glu,
c-fos, SP and IL-6 compared with OA rats (P < 0.05,
Figure 4C).