Rat model of OA
After one week of adaptive feeding, all rats were anesthetized with 10% chloral hydrate (Sigma-Aldrich, St. Louis, Missouri, USA). OA rats have injected monosodium iodoacetate (MIA, 1 mg/50μL, 50μL, Sigma-Aldrich) into the right knee intraarticularly. The control group received an intraarticular injection of an equal volume of 0.9% normal saline (NS, 50μL) [25].