Statistical analysis
All statistical analyses were conducted in R, version 4.3.3 (R
Development Core Team, 2024). A two-way ANOVA was performed to examine
the effects of AMF diversity (low vs high), GCF number (0, 1, and 6),
and their interactions on ecosystem multifunctionality and the ten
previously mentioned ecosystem functions. Additionally, a one-way ANOVA
was used to assess the effect of AMF inoculation (absence vs. presence)
on these indicators. Statistical significance was determined at p < 0.05, with Tukey’s test employed for post hoc comparisons.
Pearson correlation analysis was conducted to investigate the
relationships between soil pH and root colonization, soil pH and hyphal
length density, soil pH and spore density, respectively.