loading page

Bio-specific immobilization of enzymes on electrospun PHB nanofibers
  • +5
  • Sun Ah Jang,
  • Ji Hyun Park,
  • Hye Jin Lim,
  • Joon Young Oh,
  • Ki Hyeon Bae,
  • Kyung Jin Lee,
  • Jae-Kwang Song,
  • Dong-Myung Kim
Sun Ah Jang
Korea Research Institute of Chemical Technology

Corresponding Author:[email protected]

Author Profile
Ji Hyun Park
Korea Research Institute of Chemical Technology
Author Profile
Hye Jin Lim
Chungnam National University
Author Profile
Joon Young Oh
Korea Research Institute of Chemical Technology
Author Profile
Ki Hyeon Bae
Korea Research Institute of Chemical Technology
Author Profile
Kyung Jin Lee
Chungnam National University
Author Profile
Jae-Kwang Song
Korea Research Institute of Chemical Technology
Author Profile
Dong-Myung Kim
Chungnam National University
Author Profile

Abstract

Phasins are proteins found on the surface of natural polyhydroxyalkanoate (PHA) granules. Due to their high affinity for PHA, they can potentially be used as a fusion partner to immobilize other proteins. In this study, we investigated the immobilization of a lipase onto electrospun polyhydroxybutyrate nanofibers. Due to a superior surface area-to-volume ratio, PHB nanofibers retained much larger amounts of enzyme than conventional immobilization supports. More importantly, when used in combination with a phasin tag, the enzyme immobilized on PHB nanofibers exhibited markedly higher activity and reusability. Our approach combines the advantageous features of nanofibrous materials and the regio-specificity of biomolecular interactions for the efficient use of enzymes.
24 Apr 2020Submitted to Biotechnology and Bioengineering
24 Apr 2020Submission Checks Completed
24 Apr 2020Assigned to Editor