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Phospholipase Dα1 mediates the high-Mg2+ stress response partially through regulation of K+ homeostasis
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  • Daniela Kocourková,
  • Zuzana Krčková,
  • Přemysl Pejchar,
  • Kristyna Kroumanova,
  • Tereza Podmanicka,
  • Michal Daněk,
  • Jan Martinec
Daniela Kocourková
The Czech Academy of Sciences

Corresponding Author:[email protected]

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Zuzana Krčková
The Czech Academy of Sciences
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Přemysl Pejchar
Czech Academy of Sciences
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Kristyna Kroumanova
The Czech Academy of Sciences
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Tereza Podmanicka
The Czech Academy of Sciences
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Michal Daněk
The Czech Academy of Sciences
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Jan Martinec
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Abstract

Intracellular levels of Mg2+ are tightly regulated, as Mg2+ deficiency or excess affects normal plant growth and development. In Arabidopsis, we determined that phospholipase Dα1 (PLDα1) is involved in the stress response to high-magnesium conditions. The T-DNA insertion mutant pldα1 is hypersensitive to increased concentrations of magnesium, exhibiting reduced primary root length and fresh weight. PLDα1 activity increases rapidly after high-Mg2+ treatment, and this increase was found to be dose-dependent. Two lines harboring mutations in the HKD motif, which is essential for PLDα1 activity, displayed the same high-Mg2+ hypersensitivity of pldα1 plants. Moreover, we show that high concentrations of Mg2+ disrupt K+ homeostasis, and that transcription of K+ homeostasis-related genes CIPK9 and HAK5 is impaired in pldα1. Additionally, we found that the akt1, hak5 double mutant is hypersensitive to high-Mg2+. We conclude that in Arabidopsis, the enzyme activity of PLDα1 is vital in the response to high-Mg2+ conditions, and that PLDα1 mediates this response partially through regulation of K+ homeostasis.
02 Jun 2020Submitted to Plant, Cell & Environment
02 Jun 2020Submission Checks Completed
02 Jun 2020Assigned to Editor
03 Jun 2020Reviewer(s) Assigned
10 Jun 2020Review(s) Completed, Editorial Evaluation Pending
11 Jun 2020Editorial Decision: Revise Minor
13 Jun 20201st Revision Received
13 Jun 2020Assigned to Editor
13 Jun 2020Submission Checks Completed
14 Jun 2020Review(s) Completed, Editorial Evaluation Pending
14 Jun 2020Editorial Decision: Accept