Bio-functionalized titanium surfaces with the modified silk fibroin
carrying titanium binding motif enhanced the ossific differentiation of
MC3T3-E1
Abstract
Silk fibroin (SF) from Bombyx mori has superior properties as
both a textile and a biomaterial, and has been used to functionalize the
surfaces of various medical inorganic materials including titanium (Ti).
In this paper, we endowed SF with reversible binding ability to Ti by
embedding a titanium binding motif (minTBP-1, RKLPDA). Artificial SF
proteins were first created by conjugating gene cassettes for SF motif
(AGSGAG) and minTBP-1 motif with different ratios, which have been shown
to bind reversibly to Ti surfaces in quartz crystal microbalance
analyses. Based on these results, the functionalized SF (TiBP-SF)
containing the designed peptide
[TS[(AGSGAG)3AS]2RKLPDAS]8
was prepared from the cocoon of transgenic B. mori, which
accelerates the ossific differentiation of MC3T3-E1 cells when coated on
titanium substrates. Thus, TiBP-SF presents an alternative for endowing
the surfaces of titanium materials with osseointegration functionality,
which would allow the exploration of potential applications in the
medical field.