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A trans-dihydrocarvone-producing bacterial strain Klebsiella sp. O852 with potential use in bio-flavor production
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  • Lulu Zhang,
  • Jing-Nan Ren,
  • Gang Fan,
  • Jin He,
  • Wen Huang,
  • Ying-Ying Zhang,
  • Zhi Li,
  • Xiao Li,
  • Si-Yi Pan
Lulu Zhang
Huazhong Agriculture University

Corresponding Author:[email protected]

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Jing-Nan Ren
Huazhong Agriculture University
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Gang Fan
Huazhong Agricultural University
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Jin He
Huazhong Agricultural University
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Wen Huang
Huazhong Agriculture University
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Ying-Ying Zhang
Huazhong Agriculture University
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Zhi Li
Huazhong Agriculture University
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Xiao Li
Huazhong Agriculture University
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Si-Yi Pan
Huazhong Agriculture University
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Abstract

Celery spices are an important raw material in the production of food and cosmetic, including carveol, carvone and dihydrocarvone. In this study, a new Klebsiella sp. O852 was isolated, which was capable of converting limonene to trans-dihydrocarvone. The yields of trans-dihydrocarvone reached up to 1058 mg/L when the strain O852 was incubated using LB-M medium for 4 h at 36ºC and 150 rpm and the process was monitored for 36 h after adding 1680 mg/L limonene dissolved in ethanol. Limonene was used as a 20% (v/v) solution in the ethanol. Besides, the genome of Klebsiella sp. O852 comprised 20 contigs and 19 scaffolds. The genome size was 5.49 Mb. A total of 5218 protein-encoding genes were predicted, these genes mainly distributed in some metabolism and biosynthesis categories. Finally, several genes involved in trans-dihydrocarvone biosynthesis were further analyzed, identified and verified by quantitative real-time polymerase chain reaction (RT-qPCR) and exogenous expression. These novel genes may find value in the bioconversion of inexpensive raw materials to natural flavors and fragrances.
09 Sep 2020Submitted to Biotechnology and Bioengineering
09 Sep 2020Submission Checks Completed
09 Sep 2020Assigned to Editor