Transcription Factor Engineering Harnesses Metabolic Networks to meeting
industrial requirements in Yeast
Abstract
Yeast has been well-used as a typical microbial platform to make
fermented fine chemicals. However, various stress conditions severely
restrict the production costs and benefits. One effective way to resolve
such bottlenecks is to engineer transcription factors (TFs) to enhance
strain tolerance and production efficiency through remodeling the
transcript levels of different stress resistant genes. Here, we focus on
the recent advances in the mechanisms of yeast adaptive responses upon
stresses of heat, acetic acid and oxidants and classify them into
different modules within yeast cells. In particular, novel strategies
for the enhancement of both tolerance and yield by TFs engineering are
examined. In addition, the applications of artificial transcription
factor (ATFs)-based fabricating in metabolic fluxes optimization and
quantitative evaluation are discussed. Lastly, we discuss challenges and
potential solutions in exploiting TFs engineering and for A bio-based
economy products.