Advances in hybridoma preparation using electrofusion technology
- Jiaqian Kou,
- Jianzhong Shen ,
- zhanhui wang,
- Wenbo Yu
Abstract
As a rapidly developing cell engineering technique, cell electrofusion
has been increasingly applied in the field of hybridoma preparation in
recent years. However, electrofusion is a certain degree of difficulty
to completely replace the polyethylene glycol-mediated cell fusion. The
key elements limiting electrofusion in the field of hybridoma
preparation are practical complicated. This review summarizes the state
of art of cell electrofusion in hybridoma preparation based on recent
published literatures, mainly focusing on electrofusion instruments and
their components, process control, cell treatment, and process
characterization. The review provides new information and insightful
commentary, critically important to the promotion of further
electrofusion development in the field of hybridoma preparation.21 Aug 2022Submitted to Biotechnology Journal 22 Aug 2022Submission Checks Completed
22 Aug 2022Assigned to Editor
24 Aug 2022Reviewer(s) Assigned
04 Oct 2022Editorial Decision: Revise Major
21 Dec 20221st Revision Received
23 Dec 2022Submission Checks Completed
23 Dec 2022Assigned to Editor
05 Jan 2023Reviewer(s) Assigned
21 Jan 2023Review(s) Completed, Editorial Evaluation Pending
02 Feb 2023Editorial Decision: Revise Major
02 May 20232nd Revision Received
03 May 2023Submission Checks Completed
03 May 2023Assigned to Editor
03 May 2023Reviewer(s) Assigned
02 Jun 2023Review(s) Completed, Editorial Evaluation Pending
02 Jun 2023Editorial Decision: Revise Minor
14 Jun 20233rd Revision Received
14 Jun 2023Submission Checks Completed
14 Jun 2023Assigned to Editor
14 Jun 2023Review(s) Completed, Editorial Evaluation Pending
15 Jun 2023Reviewer(s) Assigned
30 Jun 2023Editorial Decision: Accept