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Reconstruction of tracheal window-shape defect by 3D printed polycaprolatone scaffold coated with Silk Fibroin Methacryloyl
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  • Yibo Shan,
  • Zhiming Shen,
  • Yi Lu,
  • Jianwei Zhu,
  • Fei Sun,
  • Wenxuan Chen,
  • Lei Yuan,
  • Hongcan Shi
Yibo Shan
Yangzhou University Medical college
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Zhiming Shen
Yangzhou University Medical college
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Yi Lu
Yangzhou University Medical college
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Jianwei Zhu
Yangzhou University Medical college
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Fei Sun
Yangzhou University Medical college
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Wenxuan Chen
Yangzhou University Medical college
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Lei Yuan
Yangzhou University Medical college
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Hongcan Shi
Yangzhou University Medical college

Corresponding Author:[email protected]

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Abstract

Tracheal resection and end-to-end anastomosis has been the standard clinical approach for the treatment of most airway diseases, especially invading the lower trachea or carina. However, when long-length (exceeding 2 cm in children or 5 cm in adults) tracheal circular resection is performed, tracheal replacement therapy is often required. In this study, we aimed to utilize autologous tracheal epithelia and bone marrow mesenchymal stem cells (BMSCs) as the seeding cells, utilize polycaprolactone (PCL) coated with Silk Fibroin Methacryloyl (SilMA) as the scaffold to carry the cells and Kartogenin (KGN). Firstly, SilMA with the concentration of 10%, 15% and 20% was made, and the experiment of swelling and degradation was performed. With the increase of the concentration, the swelling ratio decreased, the degradation progress slowed down. Upon the result of CCK-8 test and HE staining of 3D co-culture, the 20% SilMA was selected. Next, SilMA and the cells attached to SilMA were characterized by scanning electron microscopy (SEM). Furthermore, in vitro cytotoxicity test shows that 20% SilMA has good cytocompatibility. The hybrid scaffold was then made by PCL coated with 20% SilMA. The mechanical test shows this hybrid scaffold has better biomechanical properties. In vivo tracheal defect repair assays were done to evaluate the effect of the hybrid substitution. H&E staining, immunohistochemical (IHC) and immunofluorescence (IF) staining showed that this hybrid substitution ensured the viability, proliferation and migration of epithelium. This study is expected to provide new strategies for the fields of tracheal replacement therapy needing mechanical properties and epithelization.
26 Jan 2023Submitted to Biotechnology Journal
27 Jan 2023Submission Checks Completed
27 Jan 2023Assigned to Editor
22 Feb 2023Reviewer(s) Assigned
20 Apr 2023Review(s) Completed, Editorial Evaluation Pending
20 Apr 2023Editorial Decision: Revise Major
26 Jun 20231st Revision Received
27 Jun 2023Submission Checks Completed
27 Jun 2023Assigned to Editor
27 Jun 2023Reviewer(s) Assigned
28 Sep 2023Review(s) Completed, Editorial Evaluation Pending
28 Sep 2023Editorial Decision: Revise Major
07 Oct 20232nd Revision Received
10 Oct 2023Submission Checks Completed
10 Oct 2023Assigned to Editor
09 Nov 2023Review(s) Completed, Editorial Evaluation Pending
09 Nov 2023Editorial Decision: Accept