Self-assembly multifunctional DNA Tetrahedron for Efficient Elimination
of Antibiotic-Resistant Bacteria
Abstract
Antibiotic resistance is a major challenge in the clinical treatment of
bacterial infectious diseases. Herein, we constructed a multifunctional
DNA nanoplatform as a versatile carrier for bacteria-specific delivery
of clinical antibiotic ciprofloxacin (CIP) and classic nanoantibiotic
silver nanoparticles (AgNP). In our rational design, CIP was efficiently
loaded in the self-assembly double-bundle DNA tetrahedron through
intercalation with DNA duplex, and single-strand DNA-modified AgNP was
embedded in the cavity of the DNA tetrahedron through hybridization.
With the site-specific assembly of targeting aptamer in the well-defined
DNA tetrahedron, the bacteria-specific dual-antibiotic delivery system
exhibited excellent combined bactericidal properties. With enhanced
antibiotic accumulation through breaking the out membrane of bacteria,
the antibiotic delivery system effectively inhibited biofilm formation
and promoted the healing of infected wounds in vivo. This DNA-based
antibiotic delivery system provides a promising strategy for the
treatment of antibiotic-resistant infections.