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A new methodology for a rapid and high-throughput comparison of molecular profiles and biological activity of phytoextracts
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  • Viviana Caldeira,
  • Luana N’Dembo,
  • Rúben Araújo,
  • Cristiana P. Von Rekowski,
  • Tiago A.H. Fonseca,
  • Pedro N.S. Sampaio,
  • Cecília Calado
Viviana Caldeira
Instituto Politecnico de Lisboa Instituto Superior de Engenharia de Lisboa

Corresponding Author:[email protected]

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Luana N’Dembo
Universidade Lusofona Associacao para a Investigacao e Desenvolvimento em Cognicao e Computacao Centrada nas Pessoas
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Rúben Araújo
Instituto Politecnico de Lisboa Instituto Superior de Engenharia de Lisboa
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Cristiana P. Von Rekowski
Instituto Politecnico de Lisboa Instituto Superior de Engenharia de Lisboa
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Tiago A.H. Fonseca
Instituto Politecnico de Lisboa Instituto Superior de Engenharia de Lisboa
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Pedro N.S. Sampaio
Universidade Lusofona Associacao para a Investigacao e Desenvolvimento em Cognicao e Computacao Centrada nas Pessoas
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Cecília Calado
Instituto Politecnico de Lisboa Centro de Investigacao em Modelacao e Optimizacao de Sistemas Multifuncionais
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Abstract

To robustly discover and explore phytocompounds, it is necessary to evaluate the interrelationships between diverse variables that affect the composition of the obtained compounds mixtures, such as the plant species, plant tissue and the phytocompounds extraction process. Furthermore, it is relevant to evaluate the biological activity associated to the high diversity of biocompounds mixtures obtained along these processes, including cytotoxicity. The present work evaluates how Fourier Transform Infra-Red (FTIR) spectroscopy can be used to acquire in a simple, rapid, economic, and high-throughput mode the whole molecular fingerprint of aqueous and ethanolic extracts obtained from leaves, seeds and flowers of Cynara cardunculus, and ethanolic extracts from Matricaria chamomilla flowers. The impact of plant species, plant tissue, and extraction procedure on phytocompounds yield and whole molecular composition was evaluated. FTIR-spectroscopy was also applied to study the effect of each extract on animal cell metabolism, and to compare this activity of different extracts. FTIR-spectra were acquired in automatic mode based on a small sample volume (25 μL) on 96-wells microplate. The low reduced volumes will further reduce costs and the quantity of biological material needed for this type of analysis while enabling to increase the diversity of conditions screened to achieve. This type of assay can therefore promote the discovery of phytocompounds.
08 Aug 2023Submitted to Biotechnology and Bioengineering
16 Aug 2023Submission Checks Completed
16 Aug 2023Assigned to Editor
16 Aug 2023Review(s) Completed, Editorial Evaluation Pending
20 Aug 2023Reviewer(s) Assigned
17 Sep 2023Editorial Decision: Revise Major
14 Nov 20231st Revision Received
08 May 2024Review(s) Completed, Editorial Evaluation Pending
08 May 2024Editorial Decision: Accept