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A novel Dhillonvirus phage bearing a unique open reading frame of intergeneric origin
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  • Anastasios Vasileiadis,
  • Petros Bozidis,
  • Konstantinos Konstantinidis,
  • Nikolaos Kesesidis,
  • Louiza Potamiti,
  • Anna Kolliopoulou,
  • Apostolos Beloukas,
  • Mihalis I. Panayiotidis,
  • Konstantina Gartzonika,
  • Ioannis Karakasiliotis
Anastasios Vasileiadis
Democritus University of Thrace
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Petros Bozidis
University of Ioannina
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Konstantinos Konstantinidis
Democritus University of Thrace
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Nikolaos Kesesidis
Democritus University of Thrace
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Louiza Potamiti
The Cyprus Institute of Neurology and Genetics Clinical Genetics Clinic
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Anna Kolliopoulou
University of West Attica
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Apostolos Beloukas
University of West Attica
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Mihalis I. Panayiotidis
The Cyprus Institute of Neurology and Genetics Clinical Genetics Clinic
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Konstantina Gartzonika
University of Ioannina
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Ioannis Karakasiliotis
Democritus University of Thrace

Corresponding Author:[email protected]

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Abstract

Escherichia Coli ( E. coli) is one of the most prevalent bacteria in the gut and plays an important role in the formation of the intestinal microbiome. In this study, Escherichia phage Ioannina, a novel member of Caudoviricetes, with high lytic activity against E. coli ATCC 25922, was isolated from hospital wastewater. Escherichia phage Ioannina was highly resistant to a broad range of temperatures and alkaline pH, but was sensitive to acidic pH. Whole genome sequencing of the phage, revealed that Escherichia phage Ioannina is a novel phage within the Dhillonvirus genus. Its genome is a 45,270 bp double-stranded DNA molecule that encodes 64 putative open reading frames (ORFs). The phylogenetic analysis of open reading frames, which diversified Escherichia phage Ioannina from other dhillonviruses, indicated that ORF31 (a putative tail fiber protein) presented higher similarity to representatives of other phage families. ORF31 was significantly more related to similar ORFs belonging to the Kuravirus and Tunavirus ( Drexlerviridae) genera, indicating a possible recombination event.
03 Oct 2023Submitted to Journal of Basic Microbiology
03 Oct 2023Submission Checks Completed
03 Oct 2023Assigned to Editor
03 Oct 2023Review(s) Completed, Editorial Evaluation Pending
07 Oct 2023Reviewer(s) Assigned