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Exogenous administration of appropriate dose of IL-17A helps to improve multiple organ dysfunction and improve survival in septic mice
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  • Yonghui Liang,
  • Haining Meng,
  • Weifeng Xie,
  • Xiangqi Meng,
  • Yan Qu
Yonghui Liang
Qingdao Municipal Hospital Group
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Haining Meng
Qingdao Municipal Hospital Group
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Weifeng Xie
Qingdao Municipal Hospital Group
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Xiangqi Meng
Qingdao Municipal Hospital Group
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Yan Qu
Qingdao Municipal Hospital Group

Corresponding Author:[email protected]

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Abstract

At present, the effect of exogenous interleukin (IL) -17A in septic mice is still controversial. This study further explored the effect of exogenous IL-17A on multiple organ function and prognosis in septic mice. Mice model of sepsis was established by cecal ligation and puncture (CLP) method, and the mice were randomly divided into 8 groups according to different intervention measures: Sham+PBS, CLP+PBS, CLP+0.25μg IL-17A, CLP+0.5μg IL-17A, CLP+1μg IL-17A, CLP+2μg IL-17A, CLP+4μg IL-17A and CLP+Anti-IL-17A. Survival rates were monitored and recorded at 12-hour intervals, and the expression changes of blood routine, related inflammatory cytokines, liver and kidney function indexes of mice. Pathological injuries of lung, liver and kidney of mice in each group were detected by H&E staining, and the evaluation of bacterial translocation in vitro was performed by inoculation medium. The results showed that except for the sham-operated group, the 7-day survival rate of the mice in the CLP+1μg IL-17A group was the highest (75%). And exogenous administration of appropriate dose of IL-17A was beneficial to reduce alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine (Cre) in septic mice. The pathological damage of lung, liver and kidney tissue also can be alleviated, and the colony count of peripheral blood and spleen tissue of mice were also significantly decreased. From this, we concluded that exogenous administration of appropriate dose of IL-17A can improve the bacterial clearance ability of septic mice, and improve the multiple organ dysfunction and 7-day survival rate of the septic mice.
27 Jan 2023Submitted to Immunity, Inflammation and Disease
09 Feb 2023Submission Checks Completed
09 Feb 2023Assigned to Editor
09 Feb 2023Review(s) Completed, Editorial Evaluation Pending
13 Feb 2023Reviewer(s) Assigned
24 Mar 2023Editorial Decision: Revise Major