Aim: To discuss role and significance of SVIL protein in the occurrence and development of gastric cancer. Materials and methods: Using GSE84437 as research object and analysis by bioinformatics. In vitro study, after knockdown SVIL in MGC-803 and SGC-7901, observing cell biological activities including proliferation, apoptosis, invasion and migration by MTT, flow cytometry, transwell and wound healing assay. Results: By bioinformatics analysis, SVIL was closely correlation with gastric cancer, low expression group was significantly better compared with high expression groups in survival rate (P＜0.001); in cell experiment, with SVIL knockdown, MGC-803 and SGC-7901 cell proliferation were significantly depressed with apoptosis significantly increasing (P＜0.001), meanwhile, cells invasion and migration abilities were significantly inhibited with SVIL knockdown in MGC-803 and SGC-7901 cell lines (P＜0.001). Conclusion: SVIL overexpression might be closely correlated with gastric cancer development.