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Lycorine destabilizes lipid rafts to inhibit inflammation via LXRα signal in the lung of mice
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  • Fuhan Wang,
  • Qirui Zhang,
  • Kan Li,
  • Yao Xue,
  • Ziyi Li,
  • Xuejiao Gao
Fuhan Wang
Northeast Agricultural University
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Qirui Zhang
Northeast Agricultural University
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Kan Li
Northeast Agricultural University
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Yao Xue
Northeast Agricultural University
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Ziyi Li
Northeast Agricultural University
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Xuejiao Gao
Northeast Agricultural University

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Abstract

Background and Purpose: Lycorine is an alkaloid that was in the bulb of the genus Lycoris. It has properties of anti-inflammatory. This study aimed to investigate the molecular mechanism by which lycorine can reduce acute lung injury (ALI). Experimental Approach: ALI model was established by intranasal injection of lipopolysaccharide (LPS). In vitro, A549 cells were treated with LPS and pretreated with lycorine for 1 hour. Key Results: The results showed that lycorine reduced histopathological changes in lung, myeloperoxidase (MPO) activity, and the production of inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in mice. Lycorine dose-dependently inhibited the production of TNF-α, IL-1β, and IL-6. It also inhibited the transmission of TLR4/NF-κB passway in LPS-stimulated A549 cells. Lycorine increased cholesterol efflux through the activated LXRα-ABCA1/ABCG pathway. Lycorine has a good binding ability with LXRα. After adding the LXRα inhibitor, the anti-inflammatory effect of lycorine was eliminated. Conclusion and Implications: Lycorine can reduce ALI that was induced by lipopolysaccharide. The anti-inflammatory mechanism of lycorine is related to the up-regulation of the LXRα-ABCA1/ABCG pathway, which inhibits TLR4-mediated inflammation by increasing cholesterol efflux and reducing TLR4 transport to lipid rafts.
06 Mar 2024Submission Checks Completed
06 Mar 2024Assigned to Editor
06 Mar 2024Review(s) Completed, Editorial Evaluation Pending
22 Mar 2024Reviewer(s) Assigned