Resveratrol and Curcumin restore anxiety behavior and motor skills by
ameliorating Acetylcholinesterase activity in NaF-intoxicated mice
Abstract
Fluoride exposure results in several pathophysiological conditions
associated with neurodegenerative changes, these include anxiety, memory
loss, disturbance in cognition and motor learning, etc. Thus, the
objective of the present study was to examine the protective effect of
antioxidants Resveratrol (Resv) and Curcumin (Cur) on chronic NaF
(Sodium Fluoride,120 ppm for 30 days) induced anxiety behavior and
retarded exploratory motor activities, by using elevated plus maze
(EPM), and open field test (OFT), as a behavioral paradigm. Effects on
intracellular calcium concentration and cell viability were also studied
in vitro. Histochemistry was done to assess the effect of treatments on
Acetylcholine neurotransmitter. The data indicated that NaF-induced
anxiety-like behavior was evident from decreased exploration activity in
OFT with increased anxiety levels in EPM, whereas on providing the Resv
or Cur (30mg/kg. body weight, for 30 days) these behavioral changes and
the fibrous staining of acetylcholine neurons of brain subregions i.e.
CA1, CA3, Perirhinal, and Dorsolateral entorhinal cortex, were restored.
The beneficial effect of phytochemicals to improve neuronal connections
was validated by performing immunohistochemical staining of doublecortin
(DCX) and neuronal nuclei (NeuN), to evaluate neuronal migration and
synaptic integration in the mentioned subregions of mice brain. The
result suggests reparative potential of Resv and Cur, showing higher
neuro-modulatory potential by ameliorating AChE to relieve stress and
restoring synaptic plasticity in the cortical-hippocampal system
offering great potential as a novel preventive therapeutic agent for
anxiety and ameliorating exploratory motor activity.