Abstract
Present antimicrobial susceptibility testing technologies generate data
at a single time point over 24 hours of a day, which are considered to
represent the susceptibility profile of the antibiotic in question. By
questioning the epistemological value of the single time-point
susceptibility testing, a time-dependent antimicrobial susceptibility
study was designed. Time-dependent antimicrobial susceptibility testing
was realized at six different hours of the same day to reveal the
time-dependent variation of in vitro interaction between antibiotics and
bacterium. The study data revealed a significant daily time-dependent
oscillation in antimicrobial susceptibility testing in terms of
inhibition zone value. The bacterium seems to be most susceptible to
antibiotics in the dark phase (or transition to the dark phase) of the
day.