Molecular changes in natural antifreeze phenomena and cryopreservation
- Lingyu Shi,
- Chuanbao Zang,
- Zhicheng Liu,
- Gang Zhao
Lingyu Shi
University of Science and Technology of China Department of Electronic Engineering and Information
Author ProfileGang Zhao
University of Science and Technology of China Department of Electronic Engineering and Information
Author ProfileAbstract
In nature, many organisms exhibit remarkable freezing tolerance and
employ rich and intriguing physiological strategies to adapt to
low-temperature environments. However, cryopreservation techniques face
challenges when it comes to freezing and thawing biological tissues and
cells, such as cryo-damage caused by crystallization and cell membrane
rupture due to osmotic imbalances. In recent years, there has been
growing interest in the molecular mechanisms underlying natural freezing
tolerance and artificial cryopreservation processes. This review delves
into the adaptive mechanisms observed in freeze-tolerant species and
hibernating animals, elucidating the significance of specific genes,
proteins, and metabolic pathways for survival. Furthermore, we discuss
advancements in cryopreservation technologies, highlighting their
impacts on cellular and tissue integrity, and analyzes the adaptive
regulation of processes like glucose metabolism, miRNA expression, and
cryoprotective protein modulation. Finally, future directions for
cryopreservation are proposed.Submitted to Biotechnology and Bioengineering Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
27 Jun 2024Review(s) Completed, Editorial Evaluation Pending
30 Jun 2024Reviewer(s) Assigned
11 Aug 20241st Revision Received
12 Aug 2024Submission Checks Completed
12 Aug 2024Assigned to Editor
12 Aug 2024Review(s) Completed, Editorial Evaluation Pending
12 Aug 2024Reviewer(s) Assigned
13 Aug 2024Editorial Decision: Accept