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Waveguide Evanescent Field Fluorescence Microscopy Images of Osteoblast Cells: The effect of trypsin and image processing using TrackMate
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  • Abdollah Hassanzadeh,
  • Seyed Navid Elyasi,
  • Salah Raza Saeed,
  • Siyamand Salih,
  • Sarkew Salah Abdulkareem
Abdollah Hassanzadeh
University of Kurdistan

Corresponding Author:[email protected]

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Seyed Navid Elyasi
University of Kurdistan
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Salah Raza Saeed
Cihan University Sulaimaniya
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Siyamand Salih
Charmo University
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Sarkew Salah Abdulkareem
University of Halabja
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Abstract

Waveguide evanescent field fluorescence microscopy (WEFF) is an evanescent based microscopy utilizes a confined thin film of light, around 100 nm, to image the plasma membrane of cells attached to a waveguide. Low photobleaching and low background beside its high axial resolution allows time-lapse imaging to investigate changes in cell morphology in the presence or absence of chemical agents. Both large field of view (FOV) and uniform illumination are very important while imaging cell-substrate contacts with an evanescent field. In the current work, we demonstrate that the WEFF microscope is capable of large FOVs with a uniform illumination source and imaging over a very long time period with a simple and an inexpensive experimental setup. The interaction of the trypsin with plasma membranes of live osteoblast cells is investigated. To analyze cell images (250 images), instead of relying on manual tracking, which is time-consuming and can introduce numerous errors, we performed image processing using TrackMate to investigate the dynamic response of cells upon exposure to trypsin. This helps to save time and increase the accuracy of the analysis. The powerful tracking and analysis capabilities of the TrackMate plugin in ImageJ is used to automatically detect the cells boarder and traces each cluster of cells. The reduction in cell area is accompanied by a notable increase in mean intensity, reflecting changes in the intracellular environment. However, the background did not change during the experiment, which proves that the fluorescence material remains attached to the cell membrane and does not leak into the cell medium.
03 Jun 2024Submitted to Microscopy Research and Technique
05 Jun 2024Submission Checks Completed
05 Jun 2024Assigned to Editor
09 Jul 2024Review(s) Completed, Editorial Evaluation Pending
09 Jul 2024Reviewer(s) Assigned
23 Jul 2024Editorial Decision: Revise Major
30 Jul 20241st Revision Received
31 Jul 2024Submission Checks Completed
31 Jul 2024Assigned to Editor
31 Jul 2024Review(s) Completed, Editorial Evaluation Pending
27 Aug 2024Reviewer(s) Assigned