loading page

Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two-step substrate addition strategy
  • Bao Yingling,
  • Wu Xueqin,
  • Xiaolong Chen
Bao Yingling
Zhejiang University of Technology College of Biotechnology and Bioengineering
Author Profile
Wu Xueqin
Zhejiang University of Technology College of Biotechnology and Bioengineering
Author Profile
Xiaolong Chen
Zhejiang University of Technology College of Biotechnology and Bioengineering

Corresponding Author:[email protected]

Author Profile

Abstract

Pterostilbene (PST), a 3,5- O-methylated derivative of resveratrol (RSV), is a natural potent antioxidant produced by some plants in trace amounts as defense compounds. It exhibits various health-promoting activities, such as anticancer, antiviral, and antimicrobial effects. Large-scale biosynthesis of PST is crucial due to the challenges associated with extracting it from plants. This study aims to develop an efficient method for PST production using an engineered E. coli strain by feeding RSV as an intermediate compound. We introduced a two-step substrate addition strategy combined with immobilized RSV (IMRSV) on macroporous adsorption resin (MAR) to enhance PST production. Five MARs were selected for RSV immobilization, and the substrate additions strategy and fermentation parameters for PST synthesis were optimized. A maximum PST concentration of 402.78 ± 9.20 mg/L was achieved, representing a 239% increase over the control. The PST titer reached 395.20 ± 23.89 mg/L in a 3-L bioreactor. In conclusion, the combination of a two-step substrate adding system and IMRSV is a promising approach for the economical and industrial-scale production of PST.
12 Jul 2024Submitted to Biotechnology and Bioengineering
17 Jul 2024Submission Checks Completed
17 Jul 2024Assigned to Editor
17 Jul 2024Review(s) Completed, Editorial Evaluation Pending
22 Jul 2024Reviewer(s) Assigned
18 Aug 2024Editorial Decision: Revise Major
29 Sep 20241st Revision Received
30 Sep 2024Submission Checks Completed
30 Sep 2024Assigned to Editor
30 Sep 2024Review(s) Completed, Editorial Evaluation Pending
06 Oct 2024Editorial Decision: Accept