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The human gut metaproteomics: insights on wet and dry workflows
  • Valeria Marzano,
  • Stefano Levi Mortera,
  • Lorenza Putignani
Valeria Marzano
Bambino Gesù Children’s Hospital, IRCCS
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Stefano Levi Mortera
Bambino Gesù Children’s Hospital, IRCCS
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Lorenza Putignani
Bambino Gesu Paediatric Hospital

Corresponding Author:[email protected]

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Abstract

The human gut microbiota (GM) is a community of microorganisms that reside in the gastrointestinal tract. Recognized as a critical element of human health, the functions of the GM extend beyond gastrointestinal well-being to influence overall systemic health and susceptibility to disease. Among the other omic sciences, metaproteomics highlights additional facets that make it a highly valuable discipline in the study of GM. Indeed, it allows the protein inventory of complex microbial communities; peptides identified and quantified by liquid chromatography coupled to mass spectrometry analyses and then are mapped to the most likely proteins, taxa and functions by querying specific databases. The aim of this review was to compile comprehensive information on metaproteomic studies of the human GM, with a focus on the bacterial component, to assist newcomers in understanding the methods and types of research conducted in this field. The review outlines key steps in a metaproteomic-based study, such as protein extraction, database selection, and bioinformatic workflow. The importance of standardization is emphasized. In addition, a list of previously published studies is provided as hints for researchers interested in investigating the role of GM in health and disease states.
16 Jul 2024Submitted to PROTEOMICS
18 Jul 2024Submission Checks Completed
18 Jul 2024Assigned to Editor
18 Jul 2024Review(s) Completed, Editorial Evaluation Pending
18 Jul 2024Reviewer(s) Assigned
17 Oct 2024Editorial Decision: Revise Minor