Objectives: Ovarian cancer has a limited immunotherapeutic response; hence, this study aimed to investigate the relationship between CXC-chemokine ligand 13 ( CXCL13) expression and overall survival (OS) rate, key immune pathways, degree of immune cell infiltration, and PD-1 checkpoint blockade. Methods: A total of 703 differentially expressed genes (DEGs) were obtained from “The Cancer Genome Atlas” (TCGA) database based on the immune and stromal scores of 379 OC patients for getting the targeted gene CXCL13. The association between CXCL13 and OS in OC patients, biological function annotation of CXCL13, and its correlation with immune components were assessed. Results: The results indicated that upregulated CXCL13 expression was positively correlated with good OC patient prognosis. CXCL13 expression was associated with six immune-related pathways, ten immune cells, and PD-1 in the OC microenvironment. Moreover, high expression of CXCL13 was related to a better tumor response and a more extended tumor-stable stage after PD-1 blocking therapy in IMvigor210. Conclusions: The study concluded that CXCL13 could be a prognostic marker and a potential immunotherapy target for OC patients, especially PD-1 checkpoint blockade.