loading page

A review and practical guide for getting started with single-cell proteomics
  • +1
  • Lavender Lin,
  • Kei Webber,
  • Andikan Nwosu,
  • Ryan Kelly
Lavender Lin
Brigham Young University
Author Profile
Kei Webber
Brigham Young University
Author Profile
Andikan Nwosu
Brigham Young University
Author Profile
Ryan Kelly
Brigham Young University

Corresponding Author:[email protected]

Author Profile

Abstract

Single-cell proteomics (SCP) has advanced significantly in recent years, with new tools that have been specifically designed for preparation and analysis of single cells now commercially available to researchers. The field is sufficiently mature to be broadly accessible to any lab capable of isolating single cells and performing bulk-scale proteomic analyses. In this review, we highlight recent work in the SCP field that has significantly lowered the barrier to entry, thus providing a practical guide for those who are newly entering the SCP field. We outline the fundamental principles and report multiple paths to accomplish the key steps of a successful SCP experiment including sample preparation, separation and mass spectrometry data acquisition and analysis. We recommend that researchers start with a label-free SCP workflow, as achieving high-quality and quantitatively accurate results is more straightforward than label-based multiplexed strategies. By leveraging these accessible means, researchers can confidently perform SCP experiments and make meaningful discoveries at the single-cell level.
22 Jul 2024Submitted to PROTEOMICS
22 Jul 2024Submission Checks Completed
22 Jul 2024Assigned to Editor
22 Jul 2024Review(s) Completed, Editorial Evaluation Pending
22 Jul 2024Reviewer(s) Assigned
03 Sep 2024Editorial Decision: Revise Minor
06 Sep 2024Review(s) Completed, Editorial Evaluation Pending
06 Sep 20241st Revision Received
10 Sep 2024Editorial Decision: Accept