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Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency
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  • Pu Yuan,
  • Muhammad Usman,
  • Wenshan Liu,
  • Ashna Adhikari,
  • Chunquan Zhang,
  • Victor Njiti,
  • ye xia
Pu Yuan
The Ohio State University
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Muhammad Usman
The Ohio State University
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Wenshan Liu
The Ohio State University
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Ashna Adhikari
The Ohio State University
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Chunquan Zhang
Alcorn State University
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Victor Njiti
Alcorn State University
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ye xia
The Ohio State University

Corresponding Author:[email protected]

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Abstract

Plant gene editing technology has significantly advanced in recent years, thereby transforming both biotechnological research and agricultural practices. This review provides a comprehensive summary of recent advancements in this rapidly evolving field, showcasing significant discoveries from improved transformation efficiency to advanced construct design. The primary focus is on the maturation of the CRISPR-Cas9 system, which has emerged as a powerful tool for precise gene editing in plants. Through a detailed exploration, we elucidate the intricacies of integrating genetic modifications into plant genomes, shedding light on transport mechanisms, transformation techniques, and optimization strategies specific to CRISPR constructs. Furthermore, we explore the initiatives aimed at extending the frontiers of gene editing to non-model plant species, showcasing the growing scope of this technology. Overall, this comprehensive review highlights the significant impact of recent advancements in plant gene editing, illuminating its transformative potential in driving agricultural innovation and biotechnological progress.
24 Jul 2024Submitted to Biotechnology Journal
26 Jul 2024Submission Checks Completed
26 Jul 2024Assigned to Editor
26 Jul 2024Review(s) Completed, Editorial Evaluation Pending
28 Jul 2024Reviewer(s) Assigned
19 Aug 2024Editorial Decision: Revise Major
14 Oct 20241st Revision Received
18 Oct 2024Submission Checks Completed
18 Oct 2024Assigned to Editor
18 Oct 2024Review(s) Completed, Editorial Evaluation Pending
19 Oct 2024Reviewer(s) Assigned
31 Oct 2024Editorial Decision: Accept