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Revisiting the Briggs ancient DNA damage model: a fast maximum likelihood method to estimate postmortem damage
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  • Lei Zhao,
  • Rasmus Henriksen,
  • Abigail Ramsøe,
  • Rasmus Nielsen,
  • Thorfinn Korneliussen
Lei Zhao
University of Copenhagen Section for GeoGenetics

Corresponding Author:[email protected]

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Rasmus Henriksen
University of Copenhagen Section for GeoGenetics
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Abigail Ramsøe
University of Copenhagen Section for GeoGenetics
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Rasmus Nielsen
UC-Berkeley
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Thorfinn Korneliussen
University of Copenhagen Section for GeoGenetics
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Abstract

Motivation: One essential initial step in the analysis of ancient DNA is to authenticate that the DNA sequencing reads actually are from ancient DNA. This is done by assessing if the reads exhibit typical characteristics of postmortem damage, including cytosine deamination and nicks. We present a novel statistical method implemented in a fast multithreaded program, ngsBriggs, that enables rapid quantification of PMD by estimation of the Briggs ancient damage model parameters (Briggs parameters). Results: Using a multinomial model with maximum likelihood fit, ngsBriggs accurately estimates the parameters of the Briggs model, quantifying the PMD signal from single and double-stranded DNA regions.We extend the original Briggs model to capture PMD signals for contemporary sequencing platforms and show that ngs- Briggs accurately estimates the Briggs parameters across a variety of contamination levels. Classification of reads into ancient or modern reads, for the purpose of decontamination, is significantly more accurate using ngsBriggs than using other methods available. Furthermore, ngsBriggs is substantially faster than other state-of-the-art methods. ngsBriggs offers a practical and accurate method for researchers seeking to authenticate ancient DNA and improve the quality of their data.
Submitted to Molecular Ecology Resources
17 May 2024Review(s) Completed, Editorial Evaluation Pending
15 Aug 2024Editorial Decision: Revise Minor
13 Sep 20241st Revision Received
14 Sep 2024Submission Checks Completed
14 Sep 2024Assigned to Editor
14 Sep 2024Review(s) Completed, Editorial Evaluation Pending
20 Sep 2024Editorial Decision: Accept