Objectives - This comprehensive study investigates the diverse bacterial species responsible for upper respiratory tract infections, their impact on different age groups, clinical diagnoses, and antibiotic resistance patterns. Design -A prospective analysis was conducted to uncover the intricate connections between various bacterial infections and their corresponding effects on a large patient population. Setting -The study’s methodology involved thorough clinical assessments to monitor respiratory diseases, culminating in the classification of bacteria and their microbial growth Participants :- . Of the 1,003 participants, 514 were found to be infected, with the 16-39 age group being the most affected. The primary clinical diagnoses included acute tonsillopharyngitis, acute tonsillitis, acute pharyngitis and acute laryngitis . Main outcome measures :- The findings underscore the respiratory system’s susceptibility to infection due to its exposure to airborne microorganisms. Results :- Analysis of 1,003 cases revealed that Streptococcus mitis had the highest infection rate across all age groups, particularly in cases of cold cough and sore throat. Other common bacteria identified were Streptococcus parasanguinis and Streptococcus sanguinis. The study aimed to assess the susceptibility of these infectious microorganisms to various medications, with Vancomycin, linezolid, and bacitracin exhibiting 100% sensitivity, while carbimoxazole displayed the highest resistance at 84%. Methicillin-resistant Staphylococcus aureus was identified as the most resistant strain, with a maximum resistance of 57.86% against all tested medications. Conclusion : This comprehensive investigation provides valuable insights into the relationship between infection and bacterial strain resistance, paving the way for the development of new therapeutic strategies.