Diamonds are primary records of carbon transport in the deep mantle. Their growth is mediated by carbon-bearing fluids, but this process is challenging to study, due both to the rarity of fluid inclusions and limitations in imaging crystal growth structures and trace element abundances. We overcome some of these limitations in two ways: 1) by careful sample selection of twinned diamonds, which tend to incorporate remnants of their growth fluids in the regions next to the twinning plane; and 2) by using a custom 3D fluorescence-lifetime imaging system to map the concentration of A-center N aggregates in the diamonds using the quenching of N3 lifetime. The mapping reveals the 3D internal growth structure in high resolution, offering a marked advantage over current popular techniques such as cathodoluminescence imaging that can only image 2D diamond surfaces.The 3D analysis shows growth initially from a central nucleation site, and then subsequently in concentric layers. The layers are periodic in their A-center concentrations but are crosscut by sinuous linear features with elevated A-center concentrations oriented roughly perpendicular to the (111) twin plane. These features have a triangular cross section aligned with the (111) face’s triangular morphology, interpreted as growth spirals centered on screw dislocations, which act as nucleation sites during periods of crystal growth. Initial crystal growth is often rapid due to supersaturation of reactants in the fluid. Rapid growth tends to incorporate more incompatible elements which may explain the relatively high N (as A-centers) concentrations along these linear features. The periodic banding suggests a pulsed fluid supply and either a change in growth rate (growth starts fast, then slows and stops at the end of each pulse); or a change in fluid composition over the course of a pulse (fluid reacts, diamond precipitates, and fluid matures).Twinned diamonds often entrap clusters of micro fluid-inclusions at the twin plane during growth. Raman mapping of these inclusions reveals the presence of hydrous-silicic fluids, N-bearing phases, hydrocarbons, as well as olivine and enstatite. These inclusions clusters are likely remnants of the primary growth fluid and will help connect our observations of growth with fluid compositions over time.Plain-Language SummaryWe studied the growth of diamonds because they are one of the primary records of carbon transport deep in earth’s mantle. This study demonstrates that diamonds grow due to periodic pulses of carbon rich-fluid moving through, and then reacting with the solid rock of the mantle. We show that a new imaging method, 3D fluorescence-lifetime mapping, is a powerful tool to add to the suite of methods available for studying the growth of diamonds. By imaging diamond growth bands, we learn about trends in the episodic nature of crystallization. It helps distinguish changes in diamond composition over time and provides context for growth around inclusions. We looked at a specific type of sample that contains remnants of the diamond forming fluid, trapped in clusters of very small inclusions. The 3D fluorescence-lifetime mapping helps us determine that the inclusions were trapped early in diamond growth. Using another method, Raman spectroscopy mapping, we identify that individual micro-inclusions within the clusters have different compositions. We find fluid inclusions rich in hydrated-silica, in nitrogen and others in hydrocarbons. This work helps us to determine more about the composition and transport of the carbon-rich diamond forming fluids deep in the earth.