As humanity advances toward permanent lunar exploration and habitation, the establishment of reliable, autonomous power systems becomes a cornerstone for the success of lunar missions. Lunar microgrids, which are responsible for supplying power to habitats and critical infrastructure on the Moon, must operate with a high degree of autonomy due to the unique challenges of the lunar environment. One critical aspect of lunar power system operation is frequency regulation. Unlike Earth’s power grids, which benefit from a wide range of dynamic grid support mechanisms, lunar microgrids must manage frequency regulation autonomously due to the absence of external support and the extreme conditions on the Moon. This paper explores the importance of autonomous frequency regulation in lunar microgrids, discussing the challenges of maintaining grid stability, ensuring energy efficiency, and mitigating power fluctuations in a microgrid system. Additionally, the role of autonomous control systems, energy storage technologies, and advanced predictive algorithms in achieving effective frequency regulation on the Moon is analyzed. By examining the operational and technical requirements for lunar energy systems, this paper highlights how autonomous frequency regulation is crucial for ensuring reliable and continuous power supply for long-term lunar habitation and exploration. Through a combination of AI-driven optimization techniques, real-time monitoring, and predictive maintenance, lunar microgrids can achieve the resilience and stability required to meet the energy demands of lunar habitats, rovers, and other essential systems.