loading page

Computational Insights in DNA Methylation: Catalytic and Mechanistic Elucidations for Forming 3-Methyl Cytosine
  • +2
  • Mansour Almatarneh,
  • Ghada Kayed,
  • Mohammednoor Altarawneh ,
  • Yuming Zhao,
  • Amita Verma
Mansour Almatarneh
University of Jordan

Corresponding Author:[email protected]

Author Profile
Ghada Kayed
University of Jordan
Author Profile
Mohammednoor Altarawneh
United Arab Emirates University
Author Profile
Yuming Zhao
Memorial University of Newfoundland
Author Profile
Amita Verma
Sam Higginbottom University of Agriculture Technology and Sciences
Author Profile

Abstract

abundantly occurring methylation process at CpG island, which has been well-known as an epigenetic modification linked to many human’s diseases. Recently, another methylation approach has been discovered to show that DNA methyltransferases (DNMTs) promote the addition of methyl group at position 3 to yield 3mC. The existence of 3mC can cause severe damages to the DNA strand, such as blocking its replication, repair, and transcription, affecting its stability, and initiating a double-strand DNA break. To gain a deeper insight into the formation of 3mC, we have performed density functional theory (DFT) modeling studies at different levels of theory to clearly map out the mechanistic details for this new methylation approach. Our computed results are in harmony with pertinent experimental observations and shed light on a crucial off-target activity of DNMTs.