Metabolic flux from the chloroplast provides signals controlling
photosynthetic acclimation to cold in Arabidopsis thaliana.
- Helena Herrmann,
- Beth Dyson,
- Matthew Miller,
- Jean Marc Schwartz,
- Giles Johnson
Abstract
Photosynthesis is especially sensitive to environmental conditions and
the composition of the photosynthetic apparatus can be modulated in
response to environmental change, a process termed photosynthetic
acclimation. Previously, we identified a role for a cytosolic fumarase,
FUM2 in acclimation to low temperature in Arabidopsis thaliana. Mutant
lines lacking FUM2 were unable to acclimate their photosynthetic
apparatus to cold. Here, using gas exchange measurements and metabolite
assays of acclimating and non-acclimating plants, we show that
acclimation to low temperature results in a change in the distribution
of photosynthetically fixed carbon to different storage pools during the
day. Proteomic analysis of wild-type Col-0 Arabidopsis and of a fum2
mutant which was unable to acclimate to cold indicates that extensive
changes occurring in response to cold are affected in the mutant.
Metabolic and proteomic data were used to parameterise metabolic models.
Using an approach called flux sampling, we show how the relative export
of triose phosphate and 3-phsphoglycerate provides a signal of the
chloroplast redox state that could underly photosynthetic acclimation to
cold.20 Jul 2020Submitted to Plant, Cell & Environment 21 Jul 2020Submission Checks Completed
21 Jul 2020Assigned to Editor
24 Jul 2020Reviewer(s) Assigned
12 Aug 2020Review(s) Completed, Editorial Evaluation Pending
12 Aug 2020Editorial Decision: Revise Minor
08 Sep 20201st Revision Received
08 Sep 2020Assigned to Editor
08 Sep 2020Submission Checks Completed
09 Sep 2020Review(s) Completed, Editorial Evaluation Pending
09 Sep 2020Editorial Decision: Accept