ABSTRACT Background:Metabolic changes seen after obstructive jaundice increase the load of oxidative stress.The thiol-disulphide homeostasis (TDB) has a vital importance as oxidatice stress biomarker. Aim: The aim of present study is to investigate the dynamic thiol-disulphide homeostasis in patients diagnosed with obstructive jaundice and to compare the dynamic thiol-disulphide homeostasis with ischemia modified albumin. Study design: A total of 146 participants who were admitted to emergency department and who consisted of 69 patients and 77 healthy volunteers, were included in the study. Detailed blood samples were obtained from the patients at the time of admission. Methods: Thiol / disulphide levels were examined using a novel method developed by Erel and Neselioglu on the patients diagnosed with obstructive jaundice during the admission. Data were evaluated in computer system. Results: The levels of total thiol (p =0.025) and native thiol (p =0.023) were statistically significantly lower in the patient with obstructive jaundice than in the control group. It was observed that there was a significant negative correlation between the native thiol levels and the ALP (r =-0.262, p<0.01), urea (r =-0.313, p<0.01), total bilirubin (r =-0.388, p<0.01), direct bilirubin (r =-0.351, p<0.01) levels. At the same time, the level of disulphide (p = 0.235) was lower in the patient group than in the control group but not statistically significant. It was found that the ischemia modified albumin (IMA) values were lower in the control group than the obstructive jaundice group (p = 0.03). Conclusion: Total thiol, native thiol and IMA levels decrease in obstructive jaundice pathogenesis.

Background/aim: The aim of this study was to evaluate the effects of general anesthesia and ultrasonography-guided interscalene block on pain and oxidative stress evaluated by thiol–disulphide balance and C-reactive protein levels in patients undergoing shoulder arthroscopy. Materials and methods: A total of 42 patients aged 18–75 years who were scheduled to undergo shoulder arthroscopy were randomized into interscalene block group (Group-IB, n = 20) and general anesthesia group (Group-GA, n = 22). All patients received patient-controlled analgesia during the postoperative period. Additional analgesics were administered to patients with a visual analog scale score of > 4. Native -thiol, total -thiol, disulphide and C-reactive protein levels were measured. Patients’ visual analog scale scores, morphine and additional analgesic consumption were recorded. A shift in thiol–disulphide balance toward decreased thiol and increased disulphide levels was regarded as an indicator of oxidative stress. Results: Pain level, morphine and additional analgesic consumption were higher in Group-GA. Native-thiol and total-thiol levels were higher in Group-IB postoperatively and also disulphide levels were lower at postoperative 18 hours. C-reactive protein levels were similar in both the groups. Conclusion: Interscalene block induced less oxidative stress during the postoperative period, as evaluated by thiol–disulphide balance.

Introduction: This study aims to determine the use of thiol/disulphide homeostasis parameters together with procalcitonin (PCT), C-reactive protein (CRP) and Multinational Association for Supportive Care in Cancer (MASCC) risk scoring system for the prediction of prognosis and mortality in the patients with febrile neutropenia presenting to the emergency department. Material and methods: The study was carried out prospectively on 53 patients with febrile neutropenia and 51 healthy subjects presenting to the emergency department. Thiol/disulphide homeostasis parameters, which are oxidative stress markers, were measured through a new method developed by Erel and Neşelioğlu. PCT and CRP were also measured. Patients were grouped in to high-risk and low-risk groups in terms of prognosis and mortality through MASCC scores. Results: Mean values of disulphide/native thiol, CRP and PCT were found to be significantly higher in the patients having febrile neutropenia (p=0.029, p<0.001 and p<0.001, respectively). Mean values of disulphide/native thiol, CRP and PCT were found to be significantly higher in the high-risk patients (p=0.038, p=0.004, and p=0.002, respectively). Conclusion: The use of thiol/disulphide homeostasis parameters, PCT and CRP together with the MASCC system may be used for the prediction of the prognosis in the patients with febrile neutropenia.