Cardiotoxicity, including acute and late-onset cardiotoxicity, was a well-known adverse effect of many types of antitumor agents. Early identification of patients with cardiotoxicity was important to ensure prompt treatment and minimize toxic effects. This study compared the potential ability of multinomial technique in early detection, prediction and combined diagnosis of chemotherapy induced cardiotoxicity. We constructed a chemotherapy induced cardiotoxicity model through intraperitoneal injection bevacizumab and 5-fluorouracil in Balb/c mice. These mice were administered with electrocardiogram, conventional echocardiography, speckle tracking echocardiography, PET-CT, detection of B-type natriuretic peptide, myocardial pathology. Compared with other cardiac diagnose techniques, speckle tracking echocardiography and 18F-FDG myocardial imaging can detect myocardial injury in the early stage. The global longitudinal strain may provide a noninvasive and accurate method for the early detection of cardiotoxicity. The long axis longitudinal strain of left ventricular first decreased in the apical segment of left ventricle. The long axis radial strain of left ventricular first decreased in the posterior segment of left ventricle. The short axis radial strain and circumferential strain of left ventricular first decreased in the anterior wall of left ventricle.