The fretting damage and wear debris on the dental implant-abutment interface (IAI) are unclear. In this study, fatigue cycle loading (FT) and chewing cycle loading (CW) test were applied to two implant systems, the fretting damage morphology and wear debris generation on the IAI were observed by a scanning electron microscope. The torque value of the central screw was measured by electronic torque tester. The fretting damage on the IAI was relatively slight and mainly plastic deformation in the FT group, which was more serious and mainly furrow wear in the CW group. Various forms of wear debris were generated. The removal torques were lower than its pre-tightening value in both groups, the decline and loss rate of the CW group was significantly higher. This study confirmed the critical roles of fretting damages and metal wear debris on the IAI in the implant-supported prosthesis.