Nucleic Acid Visualization Assay for Middle East Respiratory Syndrome
Coronavirus (MERS-CoV) by Targeting the UpE and N Gene
Abstract
Since its first emergence in 2012, cases of infection with Middle East
respiratory syndrome coronavirus (MERS-CoV) have continued to occur. In
this study, we present two nucleic acid visualization assays that target
the MERS-CoV UpE and N genes as a panel that combines reverse
transcription recombinase polymerase amplification with a closed
vertical flow visualization strip (RT-RPA-VF). The limit of detection
was 1.2×10^1 copies/μl for the UpE assay and 1.2 copies/μl for the N
assay. The two assays exhibited no cross-reactivity with multiple CoVs,
including the bat severe acute respiratory syndrome related coronavirus
(SARSr-CoV), the bat coronavirus HKU4, and the human coronaviruses 229E,
OC43, HKU1 and severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2). The RT-RPA-VF assay does not require sophisticated
equipment and provides rapid detection within 30 min, so it has
potential for use in surveillance and detection of MERS-CoV in
low-resource settings.