Essential Site Maintenance: Authorea-powered sites will be updated circa 15:00-17:00 Eastern on Tuesday 5 November.
There should be no interruption to normal services, but please contact us at [email protected] in case you face any issues.

loading page

Nucleic Acid Visualization Assay for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) by Targeting the UpE and N Gene
  • +20
  • Pei Huang,
  • Hongli Jin,
  • Yongkun Zhao,
  • Entao Li,
  • Feihu Yan,
  • Hang Chi,
  • Qi Wang,
  • Qiuxue Han,
  • Ruo Mo,
  • Yumeng Song,
  • Jinhao Bi,
  • Cui Jiao,
  • Wujian Li,
  • Hongbin He,
  • Hong-Mei Wang,
  • Aimin Ma,
  • Na Feng,
  • Jianzhong Wang,
  • Tiecheng Wang,
  • Songtao Yang,
  • Yuwei Gao,
  • Xianzhu Xia,
  • Hualei Wang
Pei Huang
Jilin Agricultural University

Corresponding Author:[email protected]

Author Profile
Hongli Jin
Jilin University
Author Profile
Yongkun Zhao
Academy of Military Medical Sciences
Author Profile
Entao Li
Academy of Military Medical Sciences
Author Profile
Feihu Yan
Academy of Military Medical Sciences
Author Profile
Hang Chi
Academy of Military Medical Sciences
Author Profile
Qi Wang
Academy of Military Medical Sciences
Author Profile
Qiuxue Han
Academy of Military Medical Sciences
Author Profile
Ruo Mo
Jilin Agricultural University
Author Profile
Yumeng Song
Jilin University
Author Profile
Jinhao Bi
Jilin Agricultural University
Author Profile
Cui Jiao
Jilin University
Author Profile
Wujian Li
Jilin University
Author Profile
Hongbin He
Shandong Normal University
Author Profile
Hong-Mei Wang
Shandong Normal University
Author Profile
Aimin Ma
Changchun Medical College
Author Profile
Na Feng
Academy of Military Medical Sciences
Author Profile
Jianzhong Wang
Jilin Agricultural University
Author Profile
Tiecheng Wang
Academy of Military Medical Sciences
Author Profile
Songtao Yang
Academy of Military Medical Sciences
Author Profile
Yuwei Gao
Academy of Military Medical Sciences
Author Profile
Xianzhu Xia
Academy of Military Medical Sciences
Author Profile
Hualei Wang
Jilin University
Author Profile

Abstract

Since its first emergence in 2012, cases of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) have continued to occur. In this study, we present two nucleic acid visualization assays that target the MERS-CoV UpE and N genes as a panel that combines reverse transcription recombinase polymerase amplification with a closed vertical flow visualization strip (RT-RPA-VF). The limit of detection was 1.2×10^1 copies/μl for the UpE assay and 1.2 copies/μl for the N assay. The two assays exhibited no cross-reactivity with multiple CoVs, including the bat severe acute respiratory syndrome related coronavirus (SARSr-CoV), the bat coronavirus HKU4, and the human coronaviruses 229E, OC43, HKU1 and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The RT-RPA-VF assay does not require sophisticated equipment and provides rapid detection within 30 min, so it has potential for use in surveillance and detection of MERS-CoV in low-resource settings.
01 Mar 2021Published in PLOS Neglected Tropical Diseases volume 15 issue 3 on pages e0009227. 10.1371/journal.pntd.0009227