Comparison of the efficacy of two sheep pox vaccines derived from
Bakırköy strain in cattle against lumpy skin disease
Abstract
Lumpy Skin Disease virus (LSDV) is involved in the genus
Capripoxvirus(CaPV) of the family Poxviridae. Sheep and goat pox (SGP)
vaccine is used heterologously against Lumpy Skin Disease(LSD) in
Turkey. In the presented study, it was investigated the propagation of
SGP vaccine virus that attenuated in primary lamb kidney cell, in bovine
origin MDBK cells and evaluated the immunity of vaccine against LSD in
cattle with comparison to the commercial SGP vaccine (Penpox-M) are used
in the field. 2 cattle were vaccinated with 10 doses of SGP vaccine
produced in MDBK. In efficacy study, 3 cattle were vaccinated with SGP
vaccine produced in MDBK, 3 cattle vaccinated with Penpox-M. 2 cattle
were not vaccinated as control animal. After vaccination on the day 31,
all animals were challenged with virulent Pendik strain and blood
samples were taken periodically. Logarithmic differences of the
challenge titres between vaccinated and non-vaccinated were calculated.
In safety study, clinical sign wasn’t observed in animals vaccinated
with 10 doses. It was determined that difference between the animals
vaccinated with vaccine produced in MDBK cell and unvaccinated was less
than log 2.5 and the viremia occurred in vaccinated animals. The titre
difference was higher than log 2.5 in animals vaccinated with the
Penpox-M, and viremia didn’t occur. In conclusion, this study revealed
that SGP vaccine virus was propagated in MDBK cells and it is safe to
administered in cattle but it didn’t provide fully protection, Penpox-M
provided protection against LSD.