A trans-dihydrocarvone-producing bacterial strain Klebsiella sp. O852
with potential use in bio-flavor production
Abstract
Celery spices are an important raw material in the production of food
and cosmetic, including carveol, carvone and dihydrocarvone. In this
study, a new Klebsiella sp. O852 was isolated, which was capable of
converting limonene to trans-dihydrocarvone. The yields of
trans-dihydrocarvone reached up to 1058 mg/L when the strain O852 was
incubated using LB-M medium for 4 h at 36ºC and 150 rpm and the process
was monitored for 36 h after adding 1680 mg/L limonene dissolved in
ethanol. Limonene was used as a 20% (v/v) solution in the ethanol.
Besides, the genome of Klebsiella sp. O852 comprised 20 contigs and 19
scaffolds. The genome size was 5.49 Mb. A total of 5218 protein-encoding
genes were predicted, these genes mainly distributed in some metabolism
and biosynthesis categories. Finally, several genes involved in
trans-dihydrocarvone biosynthesis were further analyzed, identified and
verified by quantitative real-time polymerase chain reaction (RT-qPCR)
and exogenous expression. These novel genes may find value in the
bioconversion of inexpensive raw materials to natural flavors and
fragrances.