Electrospinning has been widely used for the development of fibrous scaffolds for cartilage tissue engineering (TE), however their small pores significantly limit cell infiltration throughout the scaffolds, particularly in three-dimensional (3D) designs. In this endeavor, a direct incorporation of chondrocytes into the fibers mesh during the electrospinning presents itself as a promising solution by use of bio-electrospraying. Yet, for this technology to be effectively employed for cartilage TE, it is necessary to assess if chondrocytes are in any way adversely affected. So, in this work, several electrospraying experiments were performed by adjusting various operational parameters to evaluate their influence on chondrocyte viability and function. A high percentage of post-electrosprayed chondrocytes remained viable upon the exposure of an external electric field generated by low needle to collector distances and low applied voltages. No obvious differences were found with non-electrosprayed chondrocytes in terms of viability, morphology and proliferation. The data reported here further suggest that bio-electrospraying under the optimal operational conditions might be a promising alternative to the existent cell seeding techniques, promoting not only cells safe delivery to the scaffold, but also the development of highly cellularized and uniform tissue constructs for cartilage repair.