A hunt for OM45 synthetic petite interactions in Saccharomyces
cerevisiae reveals a role for Miro GTPase Gem1 in cristae structure
maintenance
- Antonina Shvetsova,
- Ali Masud,
- Laura Schneider,
- Geoffray Monteuuis,
- Ulrich Bergmann,
- Ilkka Miinalainen,
- J. Kalervo Hiltunen,
- Alexander Kastaniotis
Abstract
Om45 is a major protein of the yeast outer mitochondrial membrane under
respiratory conditions. However, the cellular role of the protein has
remained obscure. Previously, deletion mutant phenotypes have not been
found, and clear amino acid sequence similarities that would allow
inferring its functional role are not available. In this work, we
describe synthetic petite mutants of UGO1 and GEM1 that depend on the
presence of OM45 for respiratory growth, as well as the identification
of several multicopy suppressors of the synthetic petite phenotypes. In
the analysis of our mutants, we demonstrate that Om45 and Gem1 have a
collaborative role in the maintenance of mitochondrial morphology,
cristae structure, and mitochondrial DNA maintenance. A group of
multicopy suppressors rescuing the synthetic lethal phenotypes of the
mutants on non-fermentable carbon sources additionally supports this
result. Our results imply that the synthetic petite phenotypes we
observed are due to the disturbance of the inner mitochondrial membrane
and point to this mitochondrial sub-compartment as the main target of
action of Om45, Ugo1, and the yeast Miro GTPase Gem1.23 Mar 2021Submitted to MicrobiologyOpen 24 Mar 2021Submission Checks Completed
24 Mar 2021Assigned to Editor
24 Mar 2021Reviewer(s) Assigned
07 Apr 2021Review(s) Completed, Editorial Evaluation Pending
07 Apr 2021Editorial Decision: Revise Major
06 Aug 20211st Revision Received
10 Aug 2021Submission Checks Completed
10 Aug 2021Assigned to Editor
16 Aug 2021Review(s) Completed, Editorial Evaluation Pending
17 Aug 2021Reviewer(s) Assigned
26 Aug 2021Editorial Decision: Revise Minor
09 Sep 20212nd Revision Received
09 Sep 2021Submission Checks Completed
09 Sep 2021Assigned to Editor
09 Sep 2021Review(s) Completed, Editorial Evaluation Pending
09 Sep 2021Editorial Decision: Accept