Investigating the determinants of reproductive biology of fishes is an essential component of biological research. Breeding pattern was investigated to determine the impact of exotic Oreochromis niloticus on the native congeneric Oreochromis macrochir in the upper Kabompo River. Gonado-somatic index and sex ratio was used to investigate the breeding pattern in both invaded (where O. niloticus is present) and uninvaded (where O. niloticus is absent) sections of the river. Oreochromis macrochir was the only native congeneric species found in both sections. Results showed that the overall gonado-somatic index means for both sexes of O. macrochir in both sections were similar. For O. niloticus in invaded section indicated all year reproduction though reduced spawning in cold season (May-June), but with increased spawning activity in wet season (February-March). In O. macrochir, males and females were found breeding in both dry and wet seasons only, as for cold season no reproduction was recorded. Sex ratio (females: males) was 1:1.3 and 1:1.7 for O. niloticus and O. macrochir respectively, and both significantly deviated from the sex ratio of 1:1 (ꭓ2=8.42 and 9.37; p<0.05). Oreochromis niloticus formed the most abundant fish caught 221(63.5%) than O. macrochir 127(36.5%). Our study has revealed that O. niloticus was able to spawn in all seasons with 23% higher breeding population than O. macrochir, which explains the suppression in the abundance. We expect O. niloticus to invade further downstream of the Kabompo River due to natural dispersion.