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Genetically-determined variations in photosynthesis indicate roles for specific fatty acid species in chilling responses
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  • Donghee Hoh,
  • Patrick Horn,
  • Atsuko Kanazawa,
  • John E. Froehlich,
  • Jeffrey Cruz,
  • Oliver Tessmer,
  • David Hall,
  • Lina Yin,
  • Christoph Benning,
  • David M. Kramer
Donghee Hoh
Michigan State University

Corresponding Author:[email protected]

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Patrick Horn
East Carolina University
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Atsuko Kanazawa
Michigan State University
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John E. Froehlich
Michigan State University
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Jeffrey Cruz
MSU-DOE Plant Research Laboratory
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Oliver Tessmer
Michigan State University
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David Hall
Michigan State University
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Lina Yin
Northwest A&F University
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Christoph Benning
Michigan State University
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David M. Kramer
MSU-DOE Plant Research Laboratory
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Abstract

Using a population of recombinant inbred lines (RILs) cowpea (Vigna unguiculata. L. Walp), we tested for co-linkages between lipid contents and chilling responses of photosynthesis. Under low temperature conditions (19°C/13°C, day/night), we observed co-linkages between quantitative trait loci (QTL) intervals for photosynthetic light reactions and specific fatty acids, most strikingly, the thylakoid-specific fatty acid 16:1Δ3trans found exclusively in phosphatidylglycerol (PG 16:1t). By contrast, we did not observe co-associations with bulk polyunsaturated fatty acids or high-melting-point-PG (sum of PG 16:0, PG 18:0 PG 16:1t) previously thought to be involved in chilling sensitivity. These results suggest that in cowpea, chilling sensitivity is modulated by specific lipid interactions rather than bulk properties. We were able to recapitulate the predicted impact of PG 16:1t levels on photosynthetic responses at low temperature using mutants and transgenic Arabidopsis lines. Because PG 16:1t synthesis requires the activity of peroxiredoxin-Q, which is activated by H2O2 and known to be involved in redox signaling, we hypothesize that the accumulation of PG 16:1t occurs as a result of upstream effects on photosynthesis that alter redox status and production of reactive oxygen species.
22 Oct 2021Submitted to Plant, Cell & Environment
23 Oct 2021Submission Checks Completed
23 Oct 2021Assigned to Editor
31 Oct 2021Reviewer(s) Assigned
16 Dec 2021Review(s) Completed, Editorial Evaluation Pending
18 Dec 2021Editorial Decision: Revise Minor
Jun 2022Published in Plant, Cell & Environment volume 45 issue 6 on pages 1682-1697. 10.1111/pce.14313