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Global identification of full-length cassava lncRNAs unveils the role of CRIR1 in cold stress response
  • +7
  • shuxia li,
  • Zhihao Cheng,
  • Shiman Dong,
  • Zhibo Li,
  • Liangping Zou,
  • Pingjuan Zhao,
  • Xin Guo,
  • Yan Bao,
  • Wenquan Wang,
  • Ming Peng
shuxia li
Chinese Academy of Tropical Agricultural Sciences

Corresponding Author:[email protected]

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Zhihao Cheng
Chinese Academy of Tropical Agricultural Sciences
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Shiman Dong
Chinese Academy of Tropical Agricultural Sciences
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Zhibo Li
Chinese Academy of Tropical Agricultural Sciences
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Liangping Zou
Chinese Academy of Tropical Agricultural Sciences
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Pingjuan Zhao
Chinese Academy of Tropical Agricultural Sciences
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Xin Guo
Chinese Academy of Tropical Agricultural Sciences
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Yan Bao
Shanghai Jiao Tong University
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Wenquan Wang
Hainan University
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Ming Peng
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
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Abstract

Long non-coding RNAs (lncRNAs) have been considered to be important regulators of gene expression in a range of biological processes in plants. A large number of lncRNAs have been identified in plants. However, most of their biological functions still remain to be determined. Here, we identified total 3 004 lncRNAs in cassava under normal or cold-treated conditions from Iso-seq data. We further characterized a lincRNA, CRIR1, as a novel positive regulator of the plant response to cold stress. CRIR1 can be significantly induced by cold treatment. Overexpression of CRIR1 in cassava enhanced the cold tolerance of transgenic plants. Transcriptome analysis demonstrated that CRIR1 regulates a range of cold stress-related genes in a CBF-independent pathway. We further found that CRIR1 RNA can interact with MeCSP5, a homolog of the cold shock protein that acts as RNA chaperones, indicating that CRIR1 may recruit MeCSP5 to improve the translation efficiency of mRNA. In summary, our study greatly extends the repertoire of lncRNAs in plants as well as its responding to cold stress. Moreover, it reveals a sophisticated mechanism by which CRIR1 regulates plant cold stress response by modulating the expression of stress-responsive genes and increasing the translational yield.
08 Oct 2021Submitted to Plant, Cell & Environment
09 Oct 2021Submission Checks Completed
09 Oct 2021Assigned to Editor
15 Oct 2021Reviewer(s) Assigned
02 Nov 2021Review(s) Completed, Editorial Evaluation Pending
04 Nov 2021Editorial Decision: Revise Minor
09 Nov 20211st Revision Received
09 Nov 2021Submission Checks Completed
09 Nov 2021Assigned to Editor
13 Nov 2021Review(s) Completed, Editorial Evaluation Pending
13 Nov 2021Editorial Decision: Revise Minor
17 Nov 20212nd Revision Received
17 Nov 2021Submission Checks Completed
17 Nov 2021Assigned to Editor
20 Nov 2021Review(s) Completed, Editorial Evaluation Pending
20 Nov 2021Editorial Decision: Accept