Magnetic Fe-Ni bimetallic oxide shell nanorods containing anti-protein
polymer segments for protein purification
Abstract
In this paper, BSA surface-imprinted magnetic Fe-Ni bimetallic oxide
shell nanorods (m-FeNi@MIPs@PCBMA) were prepared with the assistance of
poly(3-[[2-(methacryloyloxy)ethyl]dimethylammonium] propionate
(PCBMA) in connection with the surface-imprinting technique. The Fe-Ni
bimetallic oxide shell layer nanorods (m-FeNi) with magnetic
responsiveness simplified the separation and recovery process of
adsorbed materials. The controlled introduction of PCBMA facilitated the
reduction of protein non-specific adsorption. At the optimal
encapsulation ratio of 1:0.75 (Wm-FeNi@MIPs: WCBMA), m-FeNi@MIPs@PCBMA
could adsorb 122.98 ± 5 mg/g of BSA within 80 min, and the value of the
imprinting factor (IF) was also increased from 1.68 (m-FeNi@MIPs) to
3.95. In the mixed protein adsorption and real sample separation
experiments, m-FeNi@MIPs@PCBMA could selectively separate BSA.
Meanwhile, after seven adsorption-desorption experiments, the loss of
BSA adsorption by the imprinted nanorods was only 15.9%, which had good
reusability. Therefore, m-FeNi@MIPs@PCBMA has a broader application
prospect in the field of protein separation and purification.