Abstract
The artificial planting of grassland serves as the most important means
of grassland ecological restoration; however, the impact of artificial
planting on soil microbial communities is not well understood. In this
study, the evolution of the microbial community structure was studied
using 16S and ITS gene sequencing techniques, and the microbial
community differences between different forage grasses were analyzed,
including different density cropping schemes, multi-year degraded
grassland and natural grassland. It was found that the high-density
planting scheme of multiple pastures exerts a great impact on soil
nutrients as well as on the soil microbial community, effectively
increasing the relative abundance of Actinobacteria and Basidiomycota,
while the microbial community structure was found to be similar to that
of natural grassland. However, in artificial planting treatment, the key
node microflora group was noted to be bacteria, which was different from
that in natural grassland, in which the key node microflora group was
fungi. In comparison, fungi were found to be more sensitive than
bacteria to different plantings.The rise in soil fungal diversity did
not improve phosphate mineralization.Overall, this study may contribute
to understanding the influence of artificial grassland on soil
properties as well as the succession of microbial communities, How to
accelerate the succession process of grassland ecosystem. which are of
great significance in promoting artificial technology to restore the
ecological environment.