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Food-sensitized children with juvenile polyps show a unique microbial signature linked to inflammation and colorectal cancer
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  • Manuela Ilid,
  • Julián Vaccaro,
  • Belén Polo,
  • Viviana Bernedo,
  • Paula Borobia,
  • Luciana Guzmán,
  • Lorena Menendez,
  • Anabella Zosi,
  • Cecilia Zubirí,
  • Marcela García,
  • Mónica M. Collavino,
  • Guillermo Docena,
  • Renata Curciarello,
  • Luis Diambra,
  • Cecilia Muglia
Manuela Ilid
Universidad Nacional de la Plata Facultad de Ciencias Exactas
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Julián Vaccaro
Universidad Nacional de la Plata Facultad de Ciencias Exactas
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Belén Polo
Universidad Nacional de la Plata Facultad de Ciencias Exactas
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Viviana Bernedo
Hospital Interzonal General de Agudos General San Martin
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Paula Borobia
Hospital Interzonal General de Agudos General San Martin
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Luciana Guzmán
Hospital Interzonal General de Agudos General San Martin
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Lorena Menendez
Hospital Interzonal General de Agudos General San Martin
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Anabella Zosi
Hospital Interzonal General de Agudos General San Martin
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Cecilia Zubirí
Hospital Interzonal General de Agudos General San Martin
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Marcela García
Hospital Interzonal General de Agudos General San Martin
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Mónica M. Collavino
Instituto de Botanica del Nordeste
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Guillermo Docena
Universidad Nacional de la Plata Facultad de Ciencias Exactas
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Renata Curciarello
Universidad Nacional de la Plata Facultad de Ciencias Exactas
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Luis Diambra
Universidad Nacional de la Plata Centro Regional de Estudios Genomicos
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Cecilia Muglia
Universidad Nacional de la Plata Facultad de Ciencias Exactas

Corresponding Author:[email protected]

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Abstract

not-yet-known not-yet-known not-yet-known unknown Background: We have previously described that 87,6 % of children with juvenile colorectal polyp (JCP) from La Plata Children’s Hospital are sensitized to food allergens. Tissues of JCP are characterized by type 2 inflammation and the abundant presence of active sites of IgE synthesis. Objective: The aim of this work was to study a potential link between polyps and gut microbiota dysbiosis. Methods : We characterized bacterial populations in feces of food allergen-sensitized children with JCP, feces from healthy children and those associated with JCP tissues. Microbial diversity and composition were evaluated using next generation sequencing of the amplified 16S rRNA gene V3-V4 hypervariable region. Results: The principal component analysis applied to β-diversity discriminated well among sample groups. The taxonomic characterization of the bacterial communities in stool samples from food-sensitized children showed a reduced abundance of Bacteroidetes (Bacteroides) and, at the genera and species levels, an increased number of Mediterraneibacter faecis, Prevotella copri, Catenibacterium , and Prevotella hominis , Sutterella wadsworthensis and Phascolarctobacterium A succinatutens compared to feces of healthy children. We found that the microbiome associated with the polyp tissues was enriched in inflammation- and colorectal cancer associated bacteria, including Fusobacterium, Escherichia and Corynebacterium, with elevated levels of Escherichia fergussoni , Akkermansia muciniphila and Enterocloster . Conclusion: Our study highlights differences in bacterial diversity between food sensitized and healthy children’s feces, and that associated with the polyps. This is the first comprehensive description of JCP microbiota in food sensitized patients and may reveal mechanisms of allergic inflammation and polyp formation, underscoring the importance of monitoring for potential malignant lesions.