Food-sensitized children with juvenile polyps show a unique microbial
signature linked to inflammation and colorectal cancer
- Manuela Ilid,
- Julián Vaccaro,
- Belén Polo,
- Viviana Bernedo,
- Paula Borobia,
- Luciana Guzmán,
- Lorena Menendez,
- Anabella Zosi,
- Cecilia Zubirí,
- Marcela García,
- Mónica M. Collavino,
- Guillermo Docena,
- Renata Curciarello,
- Luis Diambra,
- Cecilia Muglia
Manuela Ilid
Universidad Nacional de la Plata Facultad de Ciencias Exactas
Author ProfileJulián Vaccaro
Universidad Nacional de la Plata Facultad de Ciencias Exactas
Author ProfileBelén Polo
Universidad Nacional de la Plata Facultad de Ciencias Exactas
Author ProfileViviana Bernedo
Hospital Interzonal General de Agudos General San Martin
Author ProfilePaula Borobia
Hospital Interzonal General de Agudos General San Martin
Author ProfileLuciana Guzmán
Hospital Interzonal General de Agudos General San Martin
Author ProfileLorena Menendez
Hospital Interzonal General de Agudos General San Martin
Author ProfileAnabella Zosi
Hospital Interzonal General de Agudos General San Martin
Author ProfileCecilia Zubirí
Hospital Interzonal General de Agudos General San Martin
Author ProfileMarcela García
Hospital Interzonal General de Agudos General San Martin
Author ProfileGuillermo Docena
Universidad Nacional de la Plata Facultad de Ciencias Exactas
Author ProfileRenata Curciarello
Universidad Nacional de la Plata Facultad de Ciencias Exactas
Author ProfileLuis Diambra
Universidad Nacional de la Plata Centro Regional de Estudios Genomicos
Author ProfileAbstract
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Background: We have previously
described that 87,6 % of children with juvenile colorectal polyp (JCP)
from La Plata Children’s Hospital are sensitized to food allergens.
Tissues of JCP are characterized by type 2 inflammation and the abundant
presence of active sites of IgE synthesis.
Objective: The aim of this work
was to study a potential link between polyps and gut microbiota
dysbiosis. Methods : We
characterized bacterial populations in feces of food allergen-sensitized
children with JCP, feces from healthy children and those associated with
JCP tissues. Microbial diversity and composition were evaluated using
next generation sequencing of the amplified 16S rRNA gene V3-V4
hypervariable region. Results:
The principal component analysis applied to β-diversity
discriminated well among sample groups. The taxonomic characterization
of the bacterial communities in stool samples from food-sensitized
children showed a reduced abundance of Bacteroidetes (Bacteroides) and,
at the genera and species levels, an increased number of
Mediterraneibacter faecis,
Prevotella copri,
Catenibacterium , and
Prevotella hominis ,
Sutterella wadsworthensis and
Phascolarctobacterium A succinatutens
compared to feces of healthy children. We found that the
microbiome associated with the polyp tissues was enriched in
inflammation- and colorectal cancer associated bacteria, including
Fusobacterium, Escherichia and Corynebacterium, with elevated levels of
Escherichia fergussoni ,
Akkermansia muciniphila and
Enterocloster .
Conclusion: Our study highlights
differences in bacterial diversity between food sensitized and healthy
children’s feces, and that associated with the polyps. This is the first
comprehensive description of JCP microbiota in food sensitized patients
and may reveal mechanisms of allergic inflammation and polyp formation,
underscoring the importance of monitoring for potential malignant
lesions.