Precise regulating strategy based on multi-parameter fusion analysis
applied to strengthen the coenzyme Q 10 biosynthesis of Rhodobater
sphaeroides
Abstract
Multi-parameters of physiological metabolism, environmental state, cell
morphology, and manipulated variable, have mutual influences and
constraints on coenzyme Q 10 (CoQ 10)
biosynthesis of Rhodobater sphaeroides. In this work, a
multi-parameter fusional correlation-analysis were applied to illuminate
and optimize the crucial metabolism on CoQ 10
biosynthesis. The quantitative response model for accurate living cells
concentration, morphological deformation, specific oxygen consumption
rate (Q O2), and specific product biosynthesis rate were
established, the results revealed that maintain the optimal electrical
conductivity(Cond.) and Q O2 at 9.0±0.5 mS/cm and
0.06-0.08±0.01×10 -7mmol/cell/h, respectively, by
adjusting the nutrient feeding and oxygen transfer rate, the cell’s
morphology and oxygen uptake rate were strengthened. CoQ
10 production in 50L bioreactors was successfully
improved by 35% to 3384mg/L than that of control, which would be more
powerful and effective for scale up in the large-scale production of CoQ
10.