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Precise regulating strategy based on multi-parameter fusion analysis applied to strengthen the coenzyme Q 10 biosynthesis of Rhodobater sphaeroides
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  • Bo Li,
  • Ze-jian Wang,
  • Yan Ge,
  • Jianguang Liang,
  • Zhichun Zhu,
  • Biqin Chen,
  • Dan Li,
  • Ying-Ping Zhuang
Bo Li
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering

Corresponding Author:[email protected]

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Ze-jian Wang
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Yan Ge
Changzhou University School of Petrochemical Engineering
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Jianguang Liang
Changzhou University School of Petrochemical Engineering
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Zhichun Zhu
Library of Inner Mongolia
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Biqin Chen
Library of Inner Mongolia
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Dan Li
Library of Inner Mongolia
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Ying-Ping Zhuang
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Abstract

Multi-parameters of physiological metabolism, environmental state, cell morphology, and manipulated variable, have mutual influences and constraints on coenzyme Q 10 (CoQ 10) biosynthesis of Rhodobater sphaeroides. In this work, a multi-parameter fusional correlation-analysis were applied to illuminate and optimize the crucial metabolism on CoQ 10 biosynthesis. The quantitative response model for accurate living cells concentration, morphological deformation, specific oxygen consumption rate (Q O2), and specific product biosynthesis rate were established, the results revealed that maintain the optimal electrical conductivity(Cond.) and Q O2 at 9.0±0.5 mS/cm and 0.06-0.08±0.01×10 -7mmol/cell/h, respectively, by adjusting the nutrient feeding and oxygen transfer rate, the cell’s morphology and oxygen uptake rate were strengthened. CoQ 10 production in 50L bioreactors was successfully improved by 35% to 3384mg/L than that of control, which would be more powerful and effective for scale up in the large-scale production of CoQ 10.