Essential Site Maintenance: Authorea-powered sites will be updated circa 15:00-17:00 Eastern on Tuesday 5 November.
There should be no interruption to normal services, but please contact us at [email protected] in case you face any issues.

loading page

Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
  • +10
  • Isadora F. Machado,
  • Edison S. Magalhães,
  • Ana Paula S. Poeta Silva,
  • Daniel C. A. Moraes,
  • Guilherme Arruda Cezar,
  • Mafalda P. Mil-Homens,
  • Onyekachukwu Osemeke,
  • Rodrigo Paiva,
  • Cesar A. A. Moura,
  • Phillip Gauger,
  • Giovani Trevisan,
  • Gustavo Silva,
  • Daniel Linhares
Isadora F. Machado
Iowa State University College of Veterinary Medicine

Corresponding Author:[email protected]

Author Profile
Edison S. Magalhães
Iowa State University College of Veterinary Medicine
Author Profile
Ana Paula S. Poeta Silva
Iowa State University College of Veterinary Medicine
Author Profile
Daniel C. A. Moraes
Iowa State University College of Veterinary Medicine
Author Profile
Guilherme Arruda Cezar
Iowa State University College of Veterinary Medicine
Author Profile
Mafalda P. Mil-Homens
Iowa State University College of Veterinary Medicine
Author Profile
Onyekachukwu Osemeke
Iowa State University College of Veterinary Medicine
Author Profile
Rodrigo Paiva
Iowa State University College of Veterinary Medicine
Author Profile
Cesar A. A. Moura
Iowa Select Farms Iowa Falls Iowa USA
Author Profile
Phillip Gauger
Iowa State University College of Veterinary Medicine
Author Profile
Giovani Trevisan
Iowa State University College of Veterinary Medicine
Author Profile
Gustavo Silva
Iowa State University College of Veterinary Medicine
Author Profile
Daniel Linhares
Iowa State University College of Veterinary Medicine
Author Profile

Abstract

The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2-7 days of age and weaning age respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100% and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42% and 27%), processing fluids (100% and 50%), and family oral fluids (11% and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance.
21 Sep 2022Published in Frontiers in Veterinary Science volume 9. 10.3389/fvets.2022.993442