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Advances in hybridoma preparation using electrofusion technology
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  • Jiaqian Kou,
  • Jianzhong Shen ,
  • zhanhui wang,
  • Wenbo Yu
Jiaqian Kou
China Agricultural University

Corresponding Author:[email protected]

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Jianzhong Shen
China Agricultural University
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zhanhui wang
China Agricultural University
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Wenbo Yu
China Agricultural University
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Abstract

As a rapidly developing cell engineering technique, cell electrofusion has been increasingly applied in the field of hybridoma preparation in recent years. However, electrofusion is a certain degree of difficulty to completely replace the polyethylene glycol-mediated cell fusion. The key elements limiting electrofusion in the field of hybridoma preparation are practical complicated. This review summarizes the state of art of cell electrofusion in hybridoma preparation based on recent published literatures, mainly focusing on electrofusion instruments and their components, process control, cell treatment, and process characterization. The review provides new information and insightful commentary, critically important to the promotion of further electrofusion development in the field of hybridoma preparation.
21 Aug 2022Submitted to Biotechnology Journal
22 Aug 2022Submission Checks Completed
22 Aug 2022Assigned to Editor
24 Aug 2022Reviewer(s) Assigned
04 Oct 2022Editorial Decision: Revise Major
21 Dec 20221st Revision Received
23 Dec 2022Submission Checks Completed
23 Dec 2022Assigned to Editor
05 Jan 2023Reviewer(s) Assigned
21 Jan 2023Review(s) Completed, Editorial Evaluation Pending
02 Feb 2023Editorial Decision: Revise Major
02 May 20232nd Revision Received
03 May 2023Submission Checks Completed
03 May 2023Assigned to Editor
03 May 2023Reviewer(s) Assigned
02 Jun 2023Review(s) Completed, Editorial Evaluation Pending
02 Jun 2023Editorial Decision: Revise Minor
14 Jun 20233rd Revision Received
14 Jun 2023Submission Checks Completed
14 Jun 2023Assigned to Editor
14 Jun 2023Review(s) Completed, Editorial Evaluation Pending
15 Jun 2023Reviewer(s) Assigned
30 Jun 2023Editorial Decision: Accept